Cells and Tissue Core

该核心的目标是分离和增殖原代人白细胞（单核细胞和白细胞）。 外周血淋巴细胞和维持病理组织的脑库[用于所有项目和核心]。 此外，该核心将提供鼠骨髓源性巨噬细胞、人和鼠神经元巨噬细胞， 和神经胶质细胞用于神经祖细胞和神经艾滋病中基于细胞的纳米制剂的研究。严格 对这一综合性很好的核心系统采取了质量控制措施。这些设施的实质用途如下： 在上一个供资周期向所有调查员提供了这一信息。将分离原代人白细胞 通过离心法将来自HIV-1、HIV-2和B和C型肝炎血清阴性供体的单核细胞和淋巴细胞 淘洗人神经胶质细胞（小神经胶质细胞和星形胶质细胞）和/或神经元将从胎儿组织中获得。的 来自人和小鼠脑组织的神经元、神经胶质和/或祖细胞将被纯化、表征并 供实验用（所有项目）。人才库也将通过这一核心运作。 有236个样本可用，其中包括阿尔茨海默病、HIV-1相关痴呆和其他神经退行性疾病（包括但不限于中风、帕金森病和多发性硬化症）的神经病理学诊断组织。死于与神经系统无关的疾病的患者 系统作为年龄匹配对照。这些来自脑库的成年人原代细胞和其他组织 将用于制备RNA和蛋白质样本库。用于RNA和蛋白质的脑组织裂解物 研究和用于免疫组织化学的冷冻和石蜡组织将是可用的资源。这些 将为疾病的共同终点提供数据。总的来说，这个“细胞和组织核心”将提供所有的 生物标本必要的解决方案项目的研究目标，并利用 通过这种基础设施获得的仔细控制的标本，以研究神经免疫及其 与神经退化有关

The objectives of this core are to isolate and propagate primary human leukocytes (monocytes and peripheral blood lymphocytes and maintain a brain bank of pathological tissues [for all projects and cores]. In addition, the core will provide murine bone-marrow-derived macrophages, human and murine neuronal and glial cells for studies of neural progenitors and cell-based nanoformulations in neuroAIDS. Rigorous quality control measures are in place for this well-integrated core. Substantive uses of these facilities were provided to all investigators during the previous funding cycle. Primary human leukocytes will be fractionated into monocytes and lymphocytes from HIV-1, 2 and hepatitis B and C seronegative donors by centrifugal elutriation. Human glia (microglia and astrbcytes) and/or neurons will be obtained from fetal tissues. The neurons, glia, and/or progenitor cells from human and mouse brain tissue will be purified, characterized and provided for experiments (all projects). The Brain Bank will also be operated through this core. Two hundred and thirty-six specimens are available, which include tissues with neuropathological diagnoses of Alzheimer's disease, HIV-1-associated dementia and other neurodegenerative disorders (including but not limited to stroke, Parkinson's disease, and multiple sclerosis). Patients who died of diseases not related to the nervous system serve as age-matched controls. These primary adult human cells and other tissues from Brain Bank will be utilized within to prepare RNA and protein specimen banks. Brain tissue lysates for RNA and protein investigations and frozen and paraffin tissues for immunohistochemistry will be an available resource. These will provide data for common endpoints of disease. In toto, this 'cells and tissue core' will provide all the biological specimens necessary to address research objectives of the program project and utilize the carefully controlled specimens obtained through this infrastructure to investigate neural immunity and its links to neurodegeneration.