Identification and functional analysis of renal stem cells

肾干细胞的鉴定及功能分析

• 批准号: 8129526
• 负责人: ROSEMARY V SAMPOGNA
• 金额: $ 13万
• 依托单位: COLUMBIA UNIVERSITY HEALTH SCIENCES
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-08-09 至 2013-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8129526
• 关键词: Acute Kidney Failure; Acute Kidney Tubular Necrosis; Address; Adult; Analgesics; Animal Model; Antibodies; Area; Award; Behavior; Biochemistry; Biological; Biopsy; Cell Cycle; Cells; Cellular biology; Clinical; Cues; Data; Detection; Diagnostic; Disease; Doctor of Philosophy; Embryo; End stage renal failure; Environment; Epithelial; Equilibrium; Evolution; Fellowship; Genetic Transcription; Goals; Hematopoietic stem cells; Human; Hypoxia; Injury; Internal Medicine; Investigation; Kidney; Kidney Diseases; Kidney Papilla; Kidney Part; Label; Laboratories; Lead; Life; Mediating; Medical; Mentored Clinical Scientist Development Award (K08); Methodology; Methods; Microscopy; Molecular; Molecular Biology; Morbidity - disease rate; Mus; Nephrology; Organ; Papillary; Phenotype; Physiologic pulse; Population; Prevalence; Process; Proliferating; Property; Public Health; Rattus; Regulation; Renal function; Reperfusion Injury; Research Personnel; Residencies; Role; Rosemary; Scientist; Sickle Cell Anemia; Signal Transduction; Skin; Sorting - Cell Movement; Source; Stem cells; Stimulus; Structure; Techniques; Time; Tissues; Training; Transcript; Ureteral obstruction; adult stem cell; base; body system; cell behavior; cell injury; human tissue; improved; in vivo; insight; interest; kidney cell; mortality; multipotent cell; nephrogenesis; overexpression; programs; regenerative; relating to nervous system; repaired; research study; response; restoration; skills; therapeutic development; tissue culture; tissue regeneration; tongue papilla; tool; transcription factor

DESCRIPTION (provided by applicant): This is an application for a Mentored Clinical Scientist Development Award (K08) entitled "Identification and Functional Analysis of Renal Stem Cells." The candidate holds MD and PhD degrees, has completed a residency in Internal Medicine and a fellowship in Nephrology. During this award, the candidate will acquire the background and laboratory skills required to further develop as a clinical scientist in the areas of renal disease and kidney development. The goals of the proposal are: (1) To identify a specific cell marker that is unique to label retaining cells in the kidney papilla and can be used to enrich and track this population; (2) To delineate the function of kidney label retaining cells in vivo before and after injury; and (3) To identify and delineate the function of regulatory molecules in kidney label retaining cells that mediate injury in acute tubular necrosis and obstructive nephropathy. The proposed studies utilize techniques in tissue culture, microscopy, molecular and cell biology, and this program of research and study will strengthen and advance the training of the candidate in these areas. The candidate proposes an integrated program of study and research directed toward improved understanding of reparative processes and, more specifically, the response of renal progenitor cells to injury. Indeed, an ever-increasing number of investigations demonstrate that tissue regeneration occurs throughout adult life, often in organ systems thought to be irreparable. Such studies point to the post-injury restoration of biological structure and function via cell-replacement strategies. The need for a deep understanding of stem cell behavior is central to confronting these issues. Relevance of this proposal to public health: the prevalence of acute renal failure in the hospitalized setting remains high and multiplies in the setting of increased co-morbid conditions. Moreover, diseases initially involving the renal papilla such as analgesic nephropathy and sickle cell anemia often lead to more global renal injury and can lead to end stage renal disease. Only by understanding the molecular signaling that underlies the "sensing" of renal injury as well as the initiation of repair, will we be able to initiate application of this new methodology to long-standing medical problems in nephrology.

描述（由申请人提供）： 这是指导临床科学家发展奖（K08）的申请，名为“肾细胞的识别和功能分析”。候选人拥有医学博士学位和博士学位，已完成内科医学居住和肾脏病研究金。在此奖项期间，候选人将获得作为肾脏疾病和肾脏发展领域的临床科学家进一步发展所需的背景和实验室技能。该提案的目标是：（1）识别特定的细胞标记物，该标记是肾脏乳头上保留细胞独有的特定细胞标记，可用于富集和跟踪该人群； （2）描绘受伤前后的肾脏标签在体内固定细胞的功能； （3）识别和描述调节分子在肾脏标记保留细胞中介导急性管状坏死和阻塞性肾病的损伤的细胞的功能。拟议的研究利用了组织培养，显微镜，分子和细胞生物学的技术，而研究和研究计划将加强和推进这些领域候选人的培训。 候选人提出了一项综合研究和研究计划，该计划致力于改善对修复过程的理解，更具体地说，是肾脏祖细胞对损伤的反应。的确，数量不断增加的研究表明，组织再生发生在整个成人生活中，通常在器官系统中被认为是无法弥补的。这样的研究指出了通过细胞替代策略对生物结构和功能的伤害恢复。深入了解干细胞行为的需求对于面对这些问题至关重要。 这项提案与公共卫生的相关性：住院环境中急性肾衰竭的流行率仍然很高，并且在增加合并症状况的情况下会增加。此外，最初涉及肾乳头状的疾病，例如镇痛性肾病和镰状细胞性贫血经常会导致更全球的肾脏损伤，并可能导致末期肾脏疾病。只有了解肾脏损伤感测的分子信号以及修复的启动，我们才能够将这种新方法启动到肾脏科中长期存在的医学问题上。