THE MUCOSAL DENDRITIC CELL-T CELL MILIEU AND SIV SPREAD

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 在第一项关于poly（IC）和直肠SIV传播的研究中，我们询问了TLR结扎poly（IC）是否可以预防SIV传播或改变未受保护动物的感染过程。在SIVwt攻击的同时（n=7）或之前24小时（n=7），将聚（IC）应用于猕猴的直肠上皮。作为对照，我们用SIVwt攻击4只动物而不接受聚（IC）处理，并包括用于测试病毒储备液的感染性的4只动物（总共n=8）。用聚（IC）同时或24小时预处理似乎不影响感染率（同时：7个感染中的4个，57%; 24小时前：7个感染中的4个，57%，对照：8个感染中的6个，75%）。然而，我们继续评估血浆病毒RNA随时间的差异（峰值和设定点）以及这18只动物的先天性和适应性免疫参数（记忆和幼稚T细胞亚群、调节性T细胞、抗体、DC和髓样和浆细胞样DC的比例，以及细胞因子和趋化因子的产生）。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. In the first study of poly(IC) and rectal SIV transmission, we asked whether TLR ligation by poly(IC) could prevent SIV transmission or modify the course of infection in animals that were not protected. Poly(IC) was applied to the rectal epithelium of macaques either coincident with (n=7) or 24 hours prior to (n=7) SIVwt challenge. As controls, we challenged 4 animals with SIVwt without receiving poly(IC) treatment and included the 4 animals used to test the infectivity of the virus stock (n=8 total). It does not appear that either the coincident or the 24 h pre-treatment with poly(IC) affected the infection rate (coincident: 4 of 7 infected, 57%; 24 h pre: 4 of 7 infected, 57%, control: 6 of 8 infected, 75%). However, we are continuing to evaluate differences in plasma virus RNA over time (at peak and set point) as well as innate and adaptive immune parameters (memory and na¿ve T cell subsets, regulatory T cells, antibodies, DCs and the ratio of myeloid and plasmacytoid DCs, and cytokine and chemokine production) in these 18 animals.