DE PEDIATRIC COBRE: CELL SCIENCE CORE

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 细胞科学核心对该中心所有五个项目的发展至关重要。该中心汇集了系内所有可用的细胞和分子生物学资源，并提供了在特拉华大学和特拉华生物技术研究所提供的其他服务。细胞科学核心将为细胞生物学、蛋白质生物化学和分子生物学的准备和分析研究提供服务和资源。 准备设施：该设施将提供一个集中的组织培养设施，用于制备来自人类受试者的匿名EBV非道德化淋巴母细胞系和成纤维细胞培养，以及人类研究组织和样本的银行，还将维护其他永生化细胞系，并作为这些基于CALL的试剂的银行。 分析设施：该设施将由细胞生物学资源、蛋白质生物化学资源和分子生物学分核心组成。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. A Cell Science Core is critical to the development of all five of the projects in this Center. The Core brings together all of the cell and molecular biological resources available within the department, and provides access to additional services available at the University of Delaware and the Delaware Biotechnology Institute. The Cell Science Core will provide services and resources for preparative and analytical studies for cell biology, protein biochemistry and molecular biology. Preparative Facility: This facility will provide a centralized staffed tissue culture facility for preparation of anonymized EBV-immoralized lymphoblastoid cell lines and fibroblast cultures from human subjects and banking of human research tissues and samples, and will also maintain other immortalized cell lines and serve as a bank for these call-based reagents. Analytical Facility: This facility will consist of the Cell Biology Resource, Protein Biochemistry Resource and Molecular Biology subcores.