Skin Phenotyping Core

皮肤病研究中心的皮肤表型核心提供了广泛的组织学， 免疫组织化学和分子工具来表征大体和微观形态， 突变和基因工程实验动物皮肤的功能特征和基因表达。 这一核心领域的特殊技术专长包括丰富的皮肤和毛囊形态知识。 了解基因/表型相关性的基本原理；最新的基因检测方法 专门为小鼠和人类皮肤设计的mRNA和蛋白质水平的表达研究；以及 拥有一系列原创的实验方法来研究活体皮肤生理学。《核心》 将协助中心调查人员以及哥伦比亚大学的其他研究人员评估 基因突变、药物或其他实验性疾病的表型表现或意外的皮肤效应 操控；了解皮肤/头发中涉及的基因和分子通路的可能功能 并分析研究人员感兴趣的基因的皮肤表达模式 用免疫组织化学和原位杂交法检测其mRNA和蛋白水平。此外，核心将 为调查人员提供出版物材料和方法部分的校对和建议 以及利用皮肤表型分析申请校外研究资金。皮肤表型 CORE还充当调查人员之间的沟通中心，为交流 样本、抗体、技术和想法。核心带来了在类似项目中工作的调查人员 技术结合在一起，从而降低了研究成本，提高了研究效率。

The Skin Phenotyping Core of the Skin Disease Research Center provides a wide array of histological, immunohistochemical, and molecular-based tools to characterize gross and microscopic morphology, functional features and gene expression in skin of mutant and genetically engineered laboratory animals. Special technical expertise within this core includes extensive knowledge of skin and hair follicle morphology essential for understanding the genotype/phenotype correlations; state of the art methods for gene expression studies at both mRNA and protein level specifically designed for mouse and human skin; and possession of a battery of original experimental approaches to the study of skin physiology in vivo. The Core will assist Center investigators as well as other researchers at Columbia in evaluating the significance of a phenotypic manifestation or unanticipated skin effects of a genetic mutation, drug, or other experimental manipulation; to understand the possible functions of genes and molecular pathways implicated in skin/hair phenotype development and to analyze skin expression patterns of genes of investigator's interest at the mRNA and protein level using immunohistochemistry and in situ hybridization. In addition, the Core will provide investigators with proof reading of and suggestions for Materials & Methods sections for publications and applications for extramural research funding utilizing skin phenotype analysis. The Skin Phenotyping Core also serves as a center of communication between investigators providing the ground for exchange of samples, antibodies, techniques and ideas. The Core brings investigators working in similar projects and techniques together thus reducing the costs and increasing efficiency of research.