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FLOW CYTOMETRY RESOURCE

流式细胞术资源

基本信息

批准号：
8114077
负责人：
ULF KLEIN
金额：
$ 16.87万
依托单位：
COLUMBIA UNIVERSITY HEALTH SCIENCES
依托单位国家：
美国
项目类别：
财政年份：
资助国家：
美国
起止时间：
至
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/8114077
关键词：
7-aminoactinomycin D Accounting Apoptosis Area Arts Biological Assay Bromodeoxyuridine Budgets Businesses Cancer Center Cancer Center Support Grant Cell Cycle Cell Separation Cell surface Cells Color Computer Workstations Computer software Computers Data Data Analyses Data Storage and Retrieval Detection Dyes Equipment Fees Flow Cytometry Flow Cytometry Shared Resource Fluorescence Fluorescence-Activated Cell Sorting Fluorochrome Funding Future Generations Genes Hour Immunophenotyping Individual Licensing Life Measurement Measures Mutation Peer Review Ploidies Population Production Propidium Diiodide Protein Analysis Proteins Research Research Personnel Resources Reverse Transcriptase Polymerase Chain Reaction Scientist Services Site Sorting - Cell Movement Speed Sterility Supervision System Techniques Training annexin A5 base cell analyzer cytokine design flexibility fluorescence activated cell sorter device instrument medical specialties member operation release of sequestered calcium ion into cytoplasm research study

项目摘要

A fully Cancer Center-managed facility, the Flow Cytometry Shared Resource assists HICCC members in flow cytometry-based studies. Services include: ¿ Fluorescence-activated cell sorting (FACS) ¿ Analytical flow cytometry for immunophenotyping ¿ Flow cytometry for analyzing cell cycle and apoptosis The capacity of the facility has increased substantially over the past three years due to the acquisition of new instruments. The facility now operates five instruments, including two fluorescence-activated cell sorters that allow high-speed cell sorting and can detect up to 12 fluorescent parameters, and one advanced cell analyzer that besides multi-color fluorescence is able to perform specialty applications such as determination of DMA content, multiple (living color) fluorescent protein analysis and calcium flux measurements. In addition to those sophisticated instruments, the facility provides access to two useroperated basic cytometers permitting 3 or 4 color immunophenotyping, respectively, as well as cell cycle and apoptosis assays. Over the last several years, the services of the facility have benefited 130 CUMC investigators, with HICCC members comprising the main users of the instruments accounting for ~60%. The high usage volume allows the facility to offer the services at low fees, which are further reduced for HICCC members. The sophisticated equipment available in the facility allows investigators at Columbia to perform state-of-the-art research in the area of flow cytometry, and therefore represent a vital resource for HICCC members. In the future, we plan to upgrade the flow cytometers to facilitate detection and sorting of newly developed living color fluorescent proteins and to purchase an instrument that serves as a flexible analysis platform for additional fluorescence-based techniques, including cytometric bead assays (CBA) to measure cytokine production, among other potential applications. During the last period of the CCSG, 36% of the investigators using the facility were Cancer Center members with peer-reviewed funding, with those members representing from 50% to 66% of the usage of the 3 available services. The proposed total operating budget of the facility is $425,004, of which we are requesting $ 116,271 from the CCSG

流式细胞术共享资源是一个完全由癌症中心管理的设施，可帮助 HICCC 成员基于流式细胞术的研究。服务包括： ¿ 荧光激活细胞分选 (FACS) ¿ 用于免疫表型分析的分析流式细胞术 ¿ 用于分析细胞周期和细胞凋亡的流式细胞术由于收购了新仪器。该设施现在运行五台仪器，其中包括两台荧光激活细胞分选仪可实现高速细胞分选并可检测多达 12 种荧光参数，以及一种先进的细胞分析仪，除了多色荧光之外，还能够执行特殊应用，例如如 DMA 含量测定、多重（活色）荧光蛋白分析和钙通量测量。除了这些复杂的仪器外，该设施还提供两个用户操作的设备基本细胞仪可分别进行 3 或 4 色免疫表型分析以及细胞周期分析和细胞凋亡测定。在过去的几年里，该设施的服务已使 130 名 CUMC 受益调查人员，HICCCC 成员是仪器的主要使用者，约占 60%。高使用量使该设施能够以低廉的费用提供服务，并进一步降低费用海商会会员。该设施中提供的先进设备使哥伦比亚的调查人员能够在流式细胞术领域进行最先进的研究，因此代表了流式细胞术的重要资源海商会会员。未来我们计划对流式细胞仪进行升级，以方便检测和分选新开发的活体彩色荧光蛋白，并购买一种作为灵活的仪器用于其他基于荧光的技术的分析平台，包括细胞计数珠测定 (CBA) 测量细胞因子的产生以及其他潜在的应用。在 CCSG 的最后一个时期，使用该设施的研究人员中有 36% 来自癌症中心拥有同行评审资金的成员，这些成员占使用量的 50% 至 66% 3 项可用服务。该设施的拟议总运营预算为 425,004 美元，其中我们向 CCSG 请求 116,271 美元