MOLECULAR CHARACTERIZATION OF THE BOCAVIRUSES

该子项目是利用 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得主要资金， 因此可以在其他CRISP条目中表示。列出的机构是 中心，不一定是研究者的机构。 背景资料：博卡病毒属是细小病毒亚科的一个新属，包括牛细小病毒（BPV）、犬细小病毒（MVC）和最近发现的人博卡病毒（HBoV）。HBoV与儿童的下呼吸道感染有关，并已被认为占全球下呼吸道感染的5%。 理由：目前，HBoV的病毒和感染性DNA都不可用，动物博卡病毒MVC的表征最终将为博卡病毒属提供一般信息。 研究问题：了解人类HBoV感染的流行病学以及博卡病毒如何在宿主细胞中复制的分子基础。结局指标：构建博卡病毒的感染性克隆，以剖析博卡病毒蛋白在病毒复制中的作用，并了解博卡病毒感染诱导受感染细胞死亡的机制。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Background: Bocavirus is a newly defined genus of subfamily Parvovirinae, which includes bovine parvovirus (BPV), minute virus of canine (MVC) and the recently identified human bocavirus (HBoV). HBoV has been associated with lower respiratory tract infections in children, and has been suggested to account for as much as 5% of lower respiratory tract infections worldwide. Rational: Currently, both virus and infectious DNA of HBoV are not available, characterization of the animal bocavirus MVC will eventually provide information to the genus Bocavirus in general. Study question: Understanding the epidemiology of HBoV infection in humans and the molecular basis how bocaviruses replicate in host cells. Outcome measures: construct infectious clone of bocaviruses to dissect the roles of bocavirus proteins in viral replication and understand the mechanism underlying bocavirus infection induces cell death of infected cells.