Liquid Chromatograph - Tandem Mass Spectrometer

液相色谱-串联质谱仪

• 批准号: 8052293
• 负责人: LOREN H. PARSONS
• 金额: $ 30.11万
• 依托单位: SCRIPPS RESEARCH INSTITUTE, THE
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-08-01 至 2012-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8052293
• 关键词: Advisory Committees; Alcohol abuse; Anxiety; Award; Biological; Brain; Characteristics; Complex; Contract Services; Detection; Drug abuse; Eating Disorders; Endocannabinoids; Funding; Health; Housing; Immunoassay; Individual; Institution; Liquid Chromatography; Liquid substance; Maintenance; Mental Depression; Mental disorders; Methods; Minor; Molecular; Noise; Pain Disorder; Peptides; Pharmaceutical Preparations; Plasma; Qualifying; Research; Research Institute; Research Personnel; Research Project Grants; Sampling; Signal Transduction; Specificity; System; Training; United States National Institutes of Health; brain tissue; capillary liquid chromatography; chronic pain; flexibility; in vivo; insight; instrument; mass spectrometer; neuromechanism; novel; operation; programs; success; tandem mass spectrometry

DESCRIPTION (provided by applicant): This is a request for a Liquid Chromatography - tandem Mass Spectrometry (LC- MS/MS) instrument that will be utilized by a major user group of five investigators at The Scripps Research Institute (TSRI) and also by a minor user group of investigators from TSRI and neighboring institutions. The requested LC-MS/MS will be used for the analysis of neuroactive peptides, endogenous cannabinoids and exogenous drugs and their metabolites in brain tissue, in vivo brain microdialysates and plasma. These analyses will directly advance research that is currently funded by NIH awards. Several characteristics of the LC-MS/MS will facilitate the research of the major and minor user groups. First, mass spectrometers provide a form of "universal detection" that offers much greater molecular specificity than is typically provided through electrochemical, spectrophotometric or immunoassay detection methods. Second, tandem mass spectrometers (MS/MS) provide substantially higher signal-to-noise ratios and considerably greater analyte specificity than do single quadrupole mass spectrometers, providing substantially lower limits of quantitation in complex biological matrices. Third, the requested capillary liquid chromatography (LC) system substantially enhances mass sensitivity and is very well suited for the separation of small volume (< 10 ?l) samples containing extremely low analyte concentrations (pM - nM) while providing flexibility for analysis of larger sample volumes as needed. The LC-MS/MS will be housed in a shared facility at TSRI that is equipped for the immediate installation of the instrument without need of renovation. TSRI has made a financial commitment to the long-term maintenance and operation of the instrument inclusive of service contracts, supplies and support of the technical staff. A highly qualified technical staff will provide user training and instrument maintenance and an Internal Advisory Committee will assure equitable instrument use by investigators both within and outside the major user group. The LC- MS/MS instrument will have a major impact on the successful completion and renewal of 22 NIH-funded research projects within the major user group that include individual R01 research projects, two NIH-funded Research Centers and one NIH-funded Program Project. Importantly, the results gathered with the requested instrument are likely to provide novel insight into the neural mechanisms contributing to drug and alcohol abuse, eating disorders, chronic pain, and psychiatric disorders such as anxiety and depression. HEALTH RELEVANCE: The requested LC-MS/MS instrument will provide the ability to quantify very low levels of neuroactive substances in biological samples including brain microdialysates. This will directly impact the progress and success of more than 22 NIH-funded research projects. The results gathered with the requested instrument will provide novel insight into the neural mechanisms contributing to drug and alcohol abuse, eating disorders, chronic pain and psychiatric disorders such as anxiety and depression.

描述(由申请人提供)： 这是对液-质联用(LC-MS/MS)仪器的请求，该仪器将由斯克里普斯研究所(TSRI)的五名研究人员组成的主要用户组以及来自TSRI和邻近机构的研究人员的一小部分用户组使用。所要求的LC-MS/MS将用于分析脑组织、活体脑微透析液和血浆中的神经活性多肽、内源性大麻素和外源性药物及其代谢物。这些分析将直接推动目前由NIH奖项资助的研究。LC-MS/MS的几个特点将有助于对主要和次要用户群体的研究。首先，质谱仪提供了一种“普遍检测”的形式，它提供了比通常通过电化学、分光光度或免疫分析检测方法提供的分子特异性要大得多的分子特异性。其次，串联质谱仪(MS/MS)提供比单一四极质谱计更高的信噪比和更高的分析物专属性，从而在复杂的生物基质中提供更低的定量极限。第三，所需的毛细管液相色谱(LC)系统大大提高了质量灵敏度，非常适合于分离含有极低分析物浓度(PM-NM)的小体积(&lt；10？L)样品，同时为根据需要分析更大体积的样品提供了灵活性。LC-MS/MS将被安置在TSRI的一个共享设施中，该设施配备有立即安装仪器的能力，无需翻新。TSRI对该文书的长期维护和运作作出了财政承诺，包括服务合同、用品和对技术人员的支持。高素质的技术人员将提供用户培训和仪器维护，内部咨询委员会将确保主要用户群体内外的调查员公平使用仪器。LC-MS/MS仪器将对主要用户群体中22个NIH资助的研究项目的成功完成和续签产生重大影响，其中包括单个R01研究项目、两个NIH资助的研究中心和一个NIH资助的计划项目。重要的是，用所要求的仪器收集的结果可能为导致药物和酒精滥用、饮食失调、慢性疼痛以及焦虑和抑郁等精神障碍的神经机制提供新的见解。 与健康相关：所要求的LC-MS/MS仪器将能够量化生物样品中非常低水平的神经活性物质，包括脑微透析液。这将直接影响NIH资助的22个以上研究项目的进展和成功。所要求的仪器收集的结果将为药物和酒精滥用、进食障碍、慢性疼痛以及焦虑和抑郁等精神障碍的神经机制提供新的见解。