Acquisition of an electron cryo-microscope for structural biology

购买用于结构生物学的电子冷冻显微镜

• 批准号: 8051881
• 负责人: Iban Ubarretxena
• 金额: $ 49.99万
• 依托单位: ICAHN SCHOOL OF MEDICINE AT MOUNT SINAI
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-04-01 至 2013-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8051881
• 关键词: Architecture; Biological; Biology; Cryoelectron Microscopy; DNA; Data Collection; Disease; Education; Electron Microscope; Electron Microscopy; Electrons; Equipment; Funding; Image; Liquid substance; Macromolecular Complexes; Membrane Proteins; Microscope; Nitrogen; Proteins; Research; Research Personnel; Role; Specimen; Structure; Techniques; Temperature; United States National Institutes of Health; charge coupled device camera; medical schools; structural biology; transmission process

DESCRIPTION (provided by applicant): Cryo-electron microscopy has gained strong prominence as a technique suitable for structural analysis of macromolecular complexes and cellular architecture. Funding is sought to acquire a state-of-the-art transmission electron cryo- microscope to establish an electron cryo-microscopy facility, devoted to research and education in structural biology, at Mount Sinai School of Medicine. The proposed equipment includes 1) a JEM-1400 transmission electron microscope with capability to image cryo-specimens; 2) cryo-transfer equipment to preserve biological specimens at liquid nitrogen temperatures and 3) a charge-coupled device (CCD) camera for data collection. This facility will be available to all Mount Sinai School of Medicine investigators, and will immediately impact vigorous NIH- funded ongoing electron microscopy efforts by several major users to study the structure of membrane proteins, protein/DNA complexes and macromolecular machines and to understand their role in biology and disease. PUBLIC HEALTH RELEVANCE: Our goal is to establish an electron cryo-microscopy facility at Mount Sinai School of Medicine to study the architecture of macromolecules and cells, and understand their role in biology and in disease.

描述（由申请人提供）：冷冻电子显微镜作为一种适用于大分子复合物和细胞结构结构分析的技术，已经获得了很高的知名度。正在寻求资金，以购买最先进的透射电子冷冻显微镜，在西奈山医学院建立一个电子冷冻显微镜设施，专门用于结构生物学的研究和教育。拟议的设备包括：（1）一台能够对低温标本成像的JEM-1400型透射电子显微镜;（2）在液氮温度下保存生物标本的低温转移设备;（3）一台用于数据收集的电荷耦合器件照相机。该设施将提供给西奈山医学院的所有研究人员，并将立即影响几个主要用户正在进行的电子显微镜研究，以研究膜蛋白，蛋白质/DNA复合物和大分子机器的结构，并了解它们在生物学和疾病中的作用。 公共卫生相关性：我们的目标是在西奈山医学院建立一个电子冷冻显微镜设施，以研究大分子和细胞的结构，并了解它们在生物学和疾病中的作用。