Acquisition of an electron cryo-microscope for structural biology

描述（由申请人提供）：冷冻电子显微镜作为一种适用于大分子复合物和细胞结构分析的技术，已经获得了很强的知名度。寻求资金购买最先进的透射电子冷冻显微镜，以建立电子冷冻显微镜设施，致力于结构生物学的研究和教育，在西奈山医学院。拟议的设备包括1)一台能够对冷冻标本成像的JEM-1400透射电子显微镜；2)冷冻转移设备，用于在液氮温度下保存生物标本；3)电荷耦合器件（CCD）相机，用于数据收集。该设施将对西奈山医学院的所有研究人员开放，并将立即影响NIH资助的几个主要用户正在进行的研究膜蛋白、蛋白质/DNA复合物和大分子机器结构的电子显微镜工作，并了解它们在生物学和疾病中的作用。

DESCRIPTION (provided by applicant): Cryo-electron microscopy has gained strong prominence as a technique suitable for structural analysis of macromolecular complexes and cellular architecture. Funding is sought to acquire a state-of-the-art transmission electron cryo- microscope to establish an electron cryo-microscopy facility, devoted to research and education in structural biology, at Mount Sinai School of Medicine. The proposed equipment includes 1) a JEM-1400 transmission electron microscope with capability to image cryo-specimens; 2) cryo-transfer equipment to preserve biological specimens at liquid nitrogen temperatures and 3) a charge-coupled device (CCD) camera for data collection. This facility will be available to all Mount Sinai School of Medicine investigators, and will immediately impact vigorous NIH- funded ongoing electron microscopy efforts by several major users to study the structure of membrane proteins, protein/DNA complexes and macromolecular machines and to understand their role in biology and disease. PUBLIC HEALTH RELEVANCE: Our goal is to establish an electron cryo-microscopy facility at Mount Sinai School of Medicine to study the architecture of macromolecules and cells, and understand their role in biology and in disease.