DNA ANALYSIS CORE FACILITY

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 DNA和RNA的分析是前沿生物医学研究的一个重要方面。为了促进应用最先进的技术来研究与得克萨斯/墨西哥边境地区有关的问题，本提案概述了DNA分析核心设施的新举措。更新的Core将现有的定量逆转录聚合酶链式反应(qRT-PCR)、数据收集和分析(UV/Vis光谱仪、凝胶记录系统和存储PhorphorImager)设备以及定制的微阵列打印、杂交和扫描设备与新获得的高容量毛细管DNA测序仪结合在一起。进行高通量基因组测序的能力将增强微阵列设计和处理方面的现有技术优势。对向BBRC研究人员、BBRC以外的UTEP调查人员以及UTEP以外的区域和国家研究人员提供的DNA测序和微阵列服务实行浮动收费标准，将使DNA分析核心设施能够过渡到自给自足的状态。核心将迁至生物科学研究大楼3楼新的、大大扩大的实验室空间，加上新的技术专门知识，将使DNA分析核心能够跟上越来越多的生物医学研究人员对其提出的需求的速度。我们提出了以下具体目标： 1.通过协助研究人员制定程序和优化DNA和蛋白质微阵列的反应条件来应对不断增加的微阵列使用 2.使用新获得的遗传分析仪制定DNA序列分析方案，并培训适当的人员使用 3.将核心改造成一个自给自足的设施，为该地区提供快速、高质量的DNA测序和微阵列服务

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The analysis of DNA and RNA is an essential aspect of cutting-edge biomedical research. In order to facilitate the application of state-of-the-art techniques to research problems relevant to the Texas/Mexico border region, this proposal outlines new initiatives for the DNA Analysis Core Facility. The updated Core combines existing equipment for quantitative reverse-transcription PCR (qRT-PCR), data collection and analysis (UV/VIS spectrophotometers, gel documentation system, and storage PhorphorImager), and custom microarray printing, hybridization and scanning equipment with a newly-acquired high capacity capillary DNA sequencer. The ability to perform high-throughput genome sequencing will augment existing technical strengths in microarray design and processing. Implementation of a sliding fee scale for DNA sequencing and microarray services provided to BBRC researchers, UTEP investigators outside the BBRC, and to regional and national researchers outside of UTEP will allow the DNA Analysis Core Facility to transition to a self-supporting status. The relocation of the Core to new, greatly expanded laboratory space on the 3rd floor of the Bioscience Research Building, together with the addition of new technical expertise, will permit the DNA Analysis Core to keep pace with the rate of demand placed upon it by an increasing number of biomedical researchers. We propose the following Specific Aims: 1. Respond to increasing microarry use by assisting researchers in developing protocols and optimizing reaction conditions for DNA and protein microarrays 2. Develop protocols for DNA sequence analysis using the newly acquired Genetic Analyzer and train appropriate personnel in its use 3. Transform the Core into a self-sustainable facility that provides the region with rapid, top-quality DNA sequencing and microarray services