CORE 4- PROTEIN BIOMARKERS CORE

这个子项目是利用资源的许多研究子项目之一 由NIH/NCRR资助的中心拨款提供。次级项目的主要支助 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， NCRR赠款不直接向子项目或子项目工作人员提供资金。 具体目标没有改变。 目标1.扩大少数民族学生、教职员工、研究项目的数量， 赠款以及受益于建设和平委员会资源和专门知识的出版物。 a）指导少数民族学生，教师和工作人员谁利用PBC。具体来说，使 设备可供他们使用，并培训他们在蛋白质组学技术，包括2- DE和毛细管液相色谱 质谱（LC/MS）和串联质谱 质谱法（LC/MS/MS）。 B）鼓励少数民族在蛋白质领域的教育、研究和发展 通过介绍同行评审的研究文章和未发表的生物标志物 在一个名为“发现蛋白质”的每月系列研讨会上工作。研讨会将在 由中国人民银行领导和主办，企业捐赠赞助。这次研讨会 系列将于2011年秋季开始。 c）在当地展示关于新型蛋白质生物标志物和方法的原创研究， 国家和国际会议，并以同行评审的方式发布这些结果 期刊 d）协助准备手稿、报告、演示文稿和提案， PBC的合作者。 e）通过寻求工具，利用区域协调员倡议对建设和平委员会的支持 仪器赠款、研究. 赠款和私人捐款。 目标二。构建PBC基础设施、工具、算法和方法。 a）安装最先进的凝胶和无凝胶毛细管LC/MS仪器， LC/MS/MS-需要从大量复杂的蛋白质中发现蛋白质生物标志物 生物样本 B）与研究者一起开发和实施最先进的凝胶， 用于蛋白质生物标志物发现的无凝胶方法。 c）结合CSB核心，设计和建立一个有效的工作流程， 每月以文件格式生成约1 TB的原始数据、峰列表和结果 特定于每个质谱仪和计算机算法制造商。 d）结合CSB Core测试开源MATLAB脚本并比较 在实施之前，使用商业计算机算法。中所述 1b）以上，这对合作研究项目尤其重要，包括 两个RCMI项目。 e）将这些进展与CSB核心结合，整合到蛋白质中 PBC的生物标志物管道。 目标3：支持RCMI研究项目。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The Specific Aims have not changed. Aim 1. Expand the number of minority students, faculty, staff, research projects, grants, and publications that benefit from the resources and expertise of the PBC. a) Mentor minority students, faculty, and staff who utilize the PBC. Specifically, make equipment available for their use and train them in proteomic techniques including 2- DE and capillary liquid chromatography mass spectrometry (LC/MS) and -tandem mass spectrometry (LC/MS/MS). b) Encourage minority education, research, and development in the field of protein biomarkers through presentation of peer-reviewed research articles and unpublished work in a monthly seminar series entitled "Discover Proteins". The seminars will be led and hosted by the PBC and sponsored by corporate donations. This seminar series will begin in Fall 2011. c) Present original research on novel protein biomarkers and methods at local, national, and international conferences, and publish these results in peer-reviewed journals. d) Assist in the preparation of manuscripts, reports, presentations, and proposals by collaborators of the PBC. e) Leverage the RCMI support for the PBC by seeking instrumentation instrumentation grants, research grants and private donations. Aim 2. Build the PBC infrastructure, instrumentation, algorithms, and methods. a) Install state-of-the-art instrumentation for gel and gel-free, capillary LC/MS and LC/MS/MS-needed to discover protein biomarkers from large numbers of complex biological samples. b) Develop and implement, in conjunction with investigators, state-of-the-art gel and gel-free methods for protein biomarker discovery. c) Design and build, in conjunction with the CSB Core, an efficient workflow to generate ~ 1 TB per month of raw data, peak lists, and results in the file format specific to each mass spectrometer and computer algorithm manufacturer. d) Test, in conjunction with the CSB Core, open-source MATLAB scripts and compare them with commercial computer algorithms prior to implementation. As described in 1b) above, this is especially important for collaborative research projects, including two RCMI projects. e) Integrate these advances, in conjunction with the CSB Core, into a protein biomarker pipeline for the PBC. Aim 3. Support the RCMI Research Projects.