Mechanims of Breast Development and Carcinogenesis

病理学核心（核心B）将通过提供人体组织， 很好。病理学中心主任Andrea Richardson博士是一名董事会认证的病理学家， 乳腺病理学专业。她是布里格姆妇女医院乳腺组织库的主任 并监督乳腺组织的收集、储存、处理和分析。乳房组织库 BWH拥有超过1500个乳腺肿瘤标本，并附有完整的病理学注释。许多标本 我经历了广泛的分子分析，包括基因表达阵列分析，SNP阵列， 基因分型，并且也在石蜡包埋的组织微阵列中呈现。除了肿瘤组织， BWH储存库还收集来自乳房缩小手术的正常乳房组织标本。这些 正常组织作为冷冻样品储存，或者可以作为新鲜样品提供， 类器官或乳腺干细胞。 病理学核心将为干细胞研究（Polyak）和3D类器官提供正常乳腺组织 文化（布鲁日）。将提供新鲜肿瘤样品以在小鼠中建立原发性肿瘤异种移植物 温伯格）。此外，Richardson博士将参与病理学比较，包括分子和 组织学，在母体人肿瘤和所得异种移植物之间。基因表达阵列数据 从人类肿瘤中获得的基因将用于BRCA 1相关通路的系统相互作用分析 （利文斯顿）。理查森博士将提供组织和科学专业知识来分析冷冻肿瘤 用于BRCA 1基因产物表达的标本和石蜡组织微阵列，包括IRIS （利文斯顿）相关性（见说明）： 使用实际的人类乳腺组织和乳腺肿瘤材料的研究将有望提供最 女性乳腺癌的发病机制和肿瘤行为的相关答案。 项目/

The Pathology Core (Core B) will support multiple projects within the program by providing human tissues for esearch. The director of the Pathology Core, Dr. Andrea Richardson, is a board certified pathologist with a specialty in breast pathology. She is director of the breast tissue bank at Brigham and Women's Hospital and oversees the collection, storage, processing and analysis of breast tissues. The breast tissue repository at BWH holds over 1500 breast tumor specimens with full pathology annotation. Many of these specimens have undergone extensive molecular analysts including gene expression array profiling, SNP array genotyping, and are also represented in paraffin-embedded tissue microarrays. In addition to tumor tissues, he BWH repository also collects normal breast tissue specimens from breast reduction surgeries. These normal tissues are stored as frozen samples or can be provided as fresh samples for isolation of normal organoids or breast stem cells. The pathology core will provide normal breast tissue for stem cell studies (Polyak) and for 3-D organoid cultures (Brugge). Fresh tumor samples will be provided to establish primary tumor xenografts in mice Weinberg). In addition, Dr. Richardson will participate in the pathologic comparisons, both molecular and histology, between the parent human tumors and the resultant xenografts. Gene expression array data obtained from human tumors will be used in system interaction analyses of BRCA1 related pathways (Livingston). Dr. Richardson will provide both tissue and scientific expertise to analyze frozen tumor specimens and paraffin tissue microarrays for the expression of BRCA1 gene products, including IRIS (Livingston).RELEVANCE (See instructions): Research using actual human breast tissue and breast tumor material will hopefully provide the most relevant answers to pathogenesis and tumor behavior of breast cancer in women. PROJECT/