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Human Tissue & Animal Core Unit

Human Tissue & Animal Core Unit
人体组织
批准号：
8198069
负责人：
John D Catravas
金额：
$38.74万
依托单位：
AUGUSTA UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2011
资助国家：
美国
项目状态：
已结题
起止时间：
2011-07-10 至 2016-06-30
来源：
https://reporter.nih.gov/project-details/8198069
关键词：
AcuteAcute Lung InjuryAdult Respiratory Distress SyndromeAnimal ModelAnimalsBiological AssayBlood capillariesCapillary PermeabilityCellsDyesEndothelial CellsEvans blue stainFunctional disorderGoalsHarvestHealthHumanInstructionLobectomyLungMeasurementMechanicsMethodsMolecularMusPermeabilityPneumonectomyProtocols documentationReproducibilityResearch DesignResearch PersonnelRespiratory physiologyScientistServicescapillaryhuman tissuein vivomortalitynovel strategiesrepairedtool

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英文摘要
Core B will have three specific aims. Specific Aim 1 is to harvest, characterize and propagate human lung microvascular endothelial cells from subjects undergoing lobectomy or pneumonectomy at MCG Health Inc.; provide said cells to investigators in all four projects for permeability, analytical and other molecular studies. Specific Aim 2 is to create and supply to all four projects mice with ALI induced by intra-tracheal instillation of gram positive or gram negative bacterial products. Specific Aim 3 is to perform in vivo lung capillary permeability (Evan's Blue Dye Method) measurements and lung function (airway mechanics) measurements for studies designed by each of the four projects.
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