EVALUATION OF ALTERNATIVE METHODS FOR MEASURING HUMAN SALIVARY AMYLASE ACTIVI

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 罗氏诊断/日立911总淀粉酶活性测定方法适用于唾液淀粉酶活性的测定。 Thermo Appliskan微板读数适合用Salimetrics法替代Dynatech Lab MRX微板读数测定唾液淀粉酶活性。 我们的第一个具体目标是比较罗氏诊断/日立911测定总淀粉酶活性的方法和利用Dynatech Lab MRX微板读取器测定唾液淀粉酶活性的Salimetrics方法，包括混合唾液和单独的唾液样本，在准确性、精密度、动态范围和方法之间的一致性方面进行比较。 我们的第二个具体目标是比较Thermo Appliskan微板读数与Dynatech Lab MRX微板读数和Salimetrics唾液淀粉酶活性测定方法的性能，利用汇集的唾液和单独的唾液样本，来比较准确度、精密度、动态范围和方法之间的一致性。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The Roche Diagnostics/Hitachi 911 method for total-amylase activity is suitable for the measurement of salivary amylase activity. The Thermo Appliskan microplate reader is a suitable substitute for the Dynatech Lab MRX microplate reader with the Salimetrics method for salivary amylase activity. Our first specific aim is to compare the Roche Diagnostics/Hitachi 911 method for total-amylase activity to the Salimetrics method for salivary amylase activity utilizing the Dynatech Lab MRX microplate reader, with both pooled saliva and individual saliva specimens, for accuracy, precision, dynamic range, and agreement between methods. Our second specific aim is to compare the performance of the Thermo Appliskan microplate reader to the performance of the Dynatech Lab MRX microplate reader with the Salimetrics method for salivary amylase activity, utilizing both pooled saliva and individual saliva specimens, for accuracy, precision, dynamic range, and agreement between methods.