Fatty Acid and Eicosanoid Analysis Core/Robert C. Murphy

脂肪酸和类二十烷酸分析核心/Robert C. Murphy

• 批准号: 8007062
• 负责人: FLOYD H CHILTON
• 金额: $ 13.45万
• 依托单位: WAKE FOREST UNIVERSITY HEALTH SCIENCES
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-07-01 至 2011-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8007062
• 关键词: Arachidonate 15-Lipoxygenase; Arachidonic Acids; Arts; Biochemical; Biological; Biological Assay; Biological Models; Blood capillaries; Botanicals; Cells; Coupled; Development; Eicosanoids; Eicosapentaenoic Acid; Event; Fatty Acids; Health; Individual; Investigation; Knowledge; Lead; Leukotrienes; Lipids; Liquid Chromatography; Mass Fragmentography; Mass Spectrum Analysis; Measures; Oils; Pathway interactions; Polyunsaturated Fatty Acids; Preventive; Prostaglandin-Endoperoxide Synthase; Prostaglandins; Research Project Grants; Sampling; Sensitivity and Specificity; Techniques; Technology; base; capillary; ionization; liquid chromatography mass spectrometry; research study; tandem mass spectrometry; tool

It has become quite clear that in order to understand the intricacies of biochemical events taking place within cells that lead to the synthesis of eicosanoids such as prostaglandins and leukotrienes, one must have a means by which one can measure virtually all products of these pathways. The development of analytical techniques have kept pace with our understanding of these needs so that extraordinarily powerful tools are now available. Mass spectrometry has emerged as the technique of choice based on sensitivity and specificity demanded by the projects proposed in this application. Advances in electro-spray ionization and tandem mass spectrometry when coupled to liquid chromatography (LC/MS), has made it possible to carry out 30 targeted eicosanoids, or more, in a single LC/MS/MS experiment. Capillary gas chromatography/mass spectrometry (GC/MS) remains an unparalleled technique in its capability to identify and quantitate fatty acids (as many a 20 fatty acids in a single experiment). Based on this state of the art technology, the major specific aims of this core are to provide state of the art assays for target lipids on a routine basis to facilitate investigations in each of the individual research projects within the Center proposal. Specifically: 1) liquid chromatography/mass spectrometry (LC/MS) and tandem mass spectrometry (LC/MS/MS) will be used to quantitate metabolites of arachidonic acid and eicosapentaenoic acid by the cyclooxygenase and 5- and 12/15-lipoxygenase pathway and 2) Gas chromatography/mass spectrometry (GC/MS) will be used to quantitate and identify fatty acids, including polyunsaturated fatty acids in biological extracts.

很明显，为了理解生物化学事件的复杂性 在导致合成类花生酸（如白藜芦醇和白三烯）的细胞内， 一种可以测量这些途径的几乎所有产物的方法。分析的发展 技术跟上了我们对这些需求的理解，因此， 现已上市。质谱法已经成为基于灵敏度和选择的技术。 本申请中提出的项目所要求的具体性。电喷雾离子化技术的研究进展 当串联质谱与液相色谱（LC/MS）偶联时，已经使得可以携带 30靶向类花生酸，或更多，在一个单一的LC/MS/MS实验。毛细管气相 色谱/质谱（GC/MS）在其鉴定能力方面仍然是无与伦比的技术， 并定量脂肪酸（在单个实验中多达20种脂肪酸）。基于目前的技术水平 技术，该核心的主要具体目标是提供针对靶脂质的最新检测方法， 常规的基础上，以促进在中心的建议内的每个单独的研究项目的调查。 具体为：1）液相色谱/质谱（LC/MS）和串联质谱 (LC/MS/MS）将用于通过 环氧合酶和5-和12/15-脂氧合酶途径和2）气相色谱/质谱 (GC/MS）将用于定量和鉴定生物样品中的脂肪酸，包括多不饱和脂肪酸。 萃取物