Analytical Core/Richard B. van Breemen

分析核心/Richard B. van Breemen

• 批准号: 8007080
• 负责人: NORMAN R FARNSWORTH
• 金额: $ 23.87万
• 依托单位: UNIVERSITY OF ILLINOIS AT CHICAGO
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-09-30 至 2015-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8007080
• 关键词: 8-Oxo-2' -Deoxyguanosine; Antioxidants; Arts; Biological; Biological Assay; Biological Availability; Biological Factors; Biological Markers; Botanicals; Carbon; Chemical Structure; Chemicals; Chemoprevention; Clinical Trials; Collaborations; Complement; Cytochrome P450; DNA; DNA Adducts; Detection; Development; Estrogen Antagonists; Estrogen Receptor Modulators; Estrogen Receptors; Estrogens; Fractionation; Funding; Human; Human Resources; Hydrolysis; In Vitro; Investigation; Laboratories; Ligands; Liquid Chromatography; Mass Spectrum Analysis; Measurement; Measures; Menopausal Symptom; Metabolic; Metabolism; Methods; Nuclear Magnetic Resonance; Oxidative Stress; Pathway interactions; Pattern; Phase; Physiologic pulse; Productivity; Progesterone; Protons; Quinone Reductases; Rattus; Research; Resolution; Response Elements; Screening procedure; Serum; Services; Standardization; Structure; Techniques; Ultrafiltration; Urine; analytical method; base; design; dietary supplements; enzyme activity; enzyme substrate; in vivo; instrument; liquid chromatography mass spectrometry; metabolic abnormality assessment; oxidation; progesterone receptor B; research study; safety study; tandem mass spectrometry

This Analytical core will be to provide essential mass spectrometry (MS) and nuclear magnetic resonance (NMR) measurements in support of all Projects and other Cores of the Botanical Center. These measurements will include routine analytical studies as well as the development of new analytical methods to support evolving research within the Center. Analytical Core personnel will provide expertise for the design and interpretation of analytical measurements for all Cores and Projects within the Center. During the first 10 years of the Botanical Center, the analytical measurements withing this core were based on mass spectrometry and have included high resolution accurate mass measurements, liquid chromatography-mass spectrometry (LC-MS), tandem mass spectrometry (MS-MS), LC-MS-MS, and pulsed ultrafiltration LC-MS (a technique invented in this laboratory for rapidly identifying ligands to pharmacological targets). In addition to providing these mass spectrometry-based analytical techniques, the analytical capabilities of this core will be expanded to include routine proton and carbon 13 ID and 2D NMR and high field NMR using 600, 800 and 900 MHz instruments. Therefore, this core will be able to provide a more complete range of analytical measurements. Several analytical assays have been developed in the projects during the previous funding period, and this core will provide these new assays as services to all Projects and Cores during the next funding period. Examples of some measurements that will be provided include high resolution 1D and 2D NMR, high resolution accurate mass LC-MS measurements for the determination of elemental compositions, MS-MS fragmentation pattern studies in support of structure elucidation in Project 1, Project 3, Core B, and Core C, and ultrafiltration-LC-MS screening assays. As needed, new MS and NMR methods will be developed and implemented for the detection, characterization and quantitative analysis of botanical natural products and their metabolites in botanical extracts and biological fiuids. In summary, this core will provide MS and NMR measurements and analytical expertise to enhance the productivity of all Projects and Cores in the Center.

该分析核心将提供必要的质谱分析（MS）和核磁共振 (NMR)测量，以支持所有项目和植物中心的其他核心。这些 测量将包括常规分析研究以及新分析方法的开发 支持中心内不断发展的研究。分析核心人员将提供专业知识， 设计和解释中心内所有岩心和项目的分析测量。期间 在植物中心的前10年，这个核心内的分析测量是基于质量 质谱法，包括高分辨率精确质量测量，液相色谱-质谱联用， 质谱法（LC-MS）、串联质谱法（MS-MS）、LC-MS-MS和脉冲超滤LC-MS（a 本实验室发明的用于快速鉴定药理学靶点配体的技术）。除了 提供这些基于质谱的分析技术，该核心的分析能力将是 扩展到包括常规质子和碳13 ID和2D NMR以及使用600、800和 900兆赫仪器。因此，这一核心将能够提供更全面的分析 测量.在上一次资助期间，在项目中开发了几种分析测定方法 该核心将在下一个时期内为所有项目和核心提供这些新的检测服务。 融资期。将提供的一些测量的示例包括高分辨率1D和2D NMR，用于测定元素组成的高分辨率精确质量LC-MS测量， MS-MS裂解模式研究，支持项目1、项目3、核心B和 核心C和超滤-LC-MS筛选测定。根据需要，将采用新的MS和NMR方法， 开发和实施的检测，表征和定量分析的植物天然 植物提取物和生物流体中的产物及其代谢产物。总而言之，该核心将提供 MS和NMR测量和分析专业知识，以提高所有项目和核心的生产力， 中心。