EXAMINATION OF LIRKO ISLETS AND SERUM USING QUANTITATIVE PROTEOMIC APPROACHES

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 糖尿病研究(1型和2型)的中心目标是为替代疗法产生大量有功能的胰岛或β细胞。因此，对促进β细胞再生的机制和因素的基础知识对于计划在体内保存和增加β细胞质量或在体外产生用于移植的β细胞是至关重要的。该项目应用定量蛋白质组学方法，在一种独特的小鼠模型-肝脏特异性胰岛素受体基因敲除(LIRKO)小鼠中，识别参与β细胞增殖的新的血液循环因子和胰岛蛋白，该小鼠的β细胞质量因胰岛素抵抗而增加20-30倍。该项目需要高灵敏度、高分辨率的LC-FTICR能力，用于对肝脏特异性胰岛素受体(LIRKO)敲除小鼠的胰岛蛋白和血清蛋白进行比较定量表征。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. A central goal of diabetes research (type 1 and type 2) is to generate large numbers of functional pancreatic islets or beta-cells for replacement therapy. Therefore, a fundamental knowledge of mechanisms and factors that promote beta-cell regeneration is essential for planning strategies to preserve and enhance beta-cell mass in vivo or to generate beta-cells in vitro for transplantation. This project applies quantitative proteomics approaches to identify new blood circulatory factors and islet proteins involved in beta-cell proliferation in a unique mouse model, the liver-specific insulin receptor knockout (LIRKO) mouse, which exhibit 20- to 30-fold increase in beta-cell mass in response to insulin resistance. This project requires high sensitivity, high resolution LC-FTICR capability from the resource for comparative quantitative characterization of the pancreatic islet proteins and serum proteins from mice with liver specific knockout of insulin receptor (LIRKO).