THREE-DIMENSIONAL ARCHITECTURES OF COMPLEX SELF-ASSEMBLING RNA PARTICLES

复杂自组装RNA颗粒的三维结构

• 批准号: 8169693
• 负责人: LUC JAEGER
• 金额: $ 2.58万
• 依托单位: SCRIPPS RESEARCH INSTITUTE, THE
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-05-01 至 2011-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8169693
• 关键词: Adopted; Architecture; Biochemical; Complex; Computer Assisted; Computer Retrieval of Information on Scientific Projects Database; Funding; Grant; Institution; Nanostructures; RNA; Research; Research Personnel; Resources; Shapes; Source; Techniques; Theoretical model; Therapeutic; United States National Institutes of Health; aptamer; engineering design; interest; nano; nanostructured; particle; scaffold

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. By taking advantage of the rich repertoire of natural RNA structural motifs, it is presently possible to generate complex artificial RNA nano-structures with interesting potential therapeutic activities. Recently, we have employed a computer-aided approach to design and engineer versatile, programmable, three-dimensional (3D) self-assembling particles made of RNA with precise control over their geometry, size and composition. These structurally complex RNA particles can be produced with good yields. As anticipated by our theoretical modeling approach, various biochemical characterization techniques suggest that these 3D particles of RNA might adopt the shape of small cubes or cuboids. However, cryo-EM characterization is necessary to validate non-ambiguously their overall 3D architectures. These RNA particles can potentially serve as nano-scaffolds for various therapeutic functions such as RNA aptamers and siRNAs. They have therefore a great potential for biomedical applications.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目和 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 通过利用丰富的天然RNA结构基序，目前可以生成具有有趣的潜在治疗活性的复杂人工RNA纳米结构。最近，我们采用计算机辅助方法来设计和制造由 RNA 制成的多功能、可编程、三维 (3D) 自组装颗粒，并精确控制其几何形状、尺寸和成分。这些结构复杂的 RNA 颗粒可以高产率生产。正如我们的理论建模方法所预期的那样，各种生化表征技术表明这些 RNA 3D 颗粒可能采用小立方体或长方体的形状。然而，冷冻电镜表征对于明确验证其整体 3D 架构是必要的。 这些 RNA 颗粒有可能作为纳米支架发挥各种治疗功能，例如 RNA 适体和 siRNA。因此，它们在生物医学应用方面具有巨大的潜力。