MECHANISMS AND INACTIVATION OF HEMOPROTEINS
血蛋白的机制和失活
基本信息
- 批准号:8169716
- 负责人:
- 金额:$ 0.71万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2010
- 资助国家:美国
- 起止时间:2010-09-12 至 2011-05-31
- 项目状态:已结题
- 来源:
- 关键词:Active SitesComputer Retrieval of Information on Scientific Projects DatabaseCytochrome P450DevelopmentEnzymesFundingGrantHemeHeme GroupHemeproteinsHorseradish PeroxidaseIndiumInstitutionInvestigationMass Spectrum AnalysisMyoglobinOxygenasesPeroxidasesProteinsResearchResearch PersonnelResourcesSkeletonSourceStructureUnited States National Institutes of Healthadductbasehemoprotein structureinhibitor/antagonistlactoperoxidaseprotein structure functionstereochemistrytool
项目摘要
This subproject is one of many research subprojects utilizing the
resources provided by a Center grant funded by NIH/NCRR. The subproject and
investigator (PI) may have received primary funding from another NIH source,
and thus could be represented in other CRISP entries. The institution listed is
for the Center, which is not necessarily the institution for the investigator.
This is a general investigation of the mechanisms of catalytic hemoproteins and of the relationships between hemoprotein structure and activity. This investigation includes, but is not limited to, studies of the cytochromes P450, horseradish peroxidase, lactoperoxidase, myoglobin, myeloperoxidase, DUOX enzymes, and heme oxygenases. A key element in the study of these proteins is the analysis of product structure, stereochemistry and isotopic substitution. The investigation of protein adducts is also important. In addition to product studies, heavy use is made of mechanism-based and other irreversible inhibitors to characterize the protein active sites. These irreversible inhibitors alkylate either the heme group or the protein. Mass spectrometry is essential for identification of the structures of the heme adducts, and consequently for the use of mechanism-based inhibitors as structural probes. The identification of protein residues that are modified by agents that react with the protein skeleton is also pursued as an additional tool for analysis of the active site structure and mechanism. These investigations, as in the past, are expected to make substantial contributions to our understanding of hemoprotein mechanisms, to the development of potentially useful hemoprotein inhibitors and to our general understanding of protein structure and function.
这个子项目是众多研究子项目之一
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
数据更新时间:{{ journalArticles.updateTime }}
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
数据更新时间:{{ journalArticles.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ monograph.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ sciAawards.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ conferencePapers.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ patent.updateTime }}
Paul R Ortiz De Montellano其他文献
Paul R Ortiz De Montellano的其他文献
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
{{ truncateString('Paul R Ortiz De Montellano', 18)}}的其他基金
LIPIDOMIC ANALYSIS OF MYCOBACTERIUM TUBERCULOSIS
结核分枝杆菌的脂质组学分析
- 批准号:
8363790 - 财政年份:2011
- 资助金额:
$ 0.71万 - 项目类别:
ROLE OF CYS RESIDUES AS A THIOL/DISULFIDE SWITCH IN HEME OXYGENASE 2 PROTEIN
半胱氨酸残基作为血红素加氧酶 2 蛋白中硫醇/二硫键开关的作用
- 批准号:
8363844 - 财政年份:2011
- 资助金额:
$ 0.71万 - 项目类别:
UNNATURAL AMINO ACID INCORPORATION INTO PROTEINS AND QUANTIFICATION THEROF
非天然氨基酸掺入蛋白质及其定量
- 批准号:
8363805 - 财政年份:2011
- 资助金额:
$ 0.71万 - 项目类别:
UNNATURAL AMINO ACID INCORPORATION INTO PROTEINS AND QUANTIFICATION THEROF
非天然氨基酸掺入蛋白质及其定量
- 批准号:
8169801 - 财政年份:2010
- 资助金额:
$ 0.71万 - 项目类别:
LIPIDOMIC ANALYSIS OF MYCOBACTERIUM TUBERCULOSIS
结核分枝杆菌的脂质组学分析
- 批准号:
8169785 - 财政年份:2010
- 资助金额:
$ 0.71万 - 项目类别:
UNNATURAL AMINO ACID INCORPORATION INTO PROTEINS AND QUANTIFICATION THEROF
非天然氨基酸掺入蛋白质及其定量
- 批准号:
7957406 - 财政年份:2009
- 资助金额:
$ 0.71万 - 项目类别:
LIPIDOMIC ANALYSIS OF MYCOBACTERIUM TUBERCULOSIS
结核分枝杆菌的脂质组学分析
- 批准号:
7957425 - 财政年份:2009
- 资助金额:
$ 0.71万 - 项目类别:
Oxygen Sensors and P450 Monooxygenases in Mycobacertium tuberculosis
结核分枝杆菌中的氧传感器和 P450 单加氧酶
- 批准号:
7294665 - 财政年份:2007
- 资助金额:
$ 0.71万 - 项目类别: