ENVELOPE PROTEIN GP140 OF HIV-1

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 囊膜蛋白gp140三聚体用SIZE排斥层析法纯化，并用SDS-PAGE、Native Gel、Western blotting等方法进行验证，sCD4和中和抗体也用SIZE-PAGE进行纯化和验证。我们希望获得一些蛋白质的小角X射线散射和衍射数据，并为免疫原设计提供这些信息。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Envelop protein gp140 trimers are purified by sizing exclusion chromatography, and verified by SDS-PAGE, Native gel, Western blotting, etc; sCD4 and neutralizing antibodies are also purified by sizing exclusion chromatography and verified by SDS-PAGE. We expect to obtain some small angle x-ray scattering and diffraction data for our proteins, and just those info for immunogen design.