PROTEOMIC ANALYSIS OF EXTINCT SPECIES TO VALIDATE EVOLUTIONARY LINKS

该子项目是利用 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 研究人员（Asara et.例如，科学2007年）已经开始使用质谱法来鉴定保存在灭绝物种（如T。雷克斯和乳齿象提供与现存物种的进化联系。这些最近的研究已经确定了胶原蛋白和血红蛋白蛋白，其含有与多种不同的现存分类群相匹配的肽序列。关于这些鉴定的肽序列是有效的还是污染的伪像存在很大争议。 我们目前正在分析六个化石恐龙骨骼样本，三个沉积物样本和一个洞熊股骨样本，看看我们可以使用我们的样品制备和质谱方法检测到什么蛋白质。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Researchers (Asara et. al., Science 2007) have started using mass spectrometry to identify proteins from fossils that have been preserved in extinct species such as T. rex and mastodon to provide evolutionary links with extant species. These recent studies have identified collagen proteins and a hemoglobin protein that contain peptide sequences that match to multiple different extant taxa. There is much controversy on whether these identified peptides sequences are valid or artifacts of contamination. We are currently analyzing six fossilized dinosaur bone samples, three sediment samples and a bone sample from the femur of a cave bear to see what proteins we can detect using our sample preparation and mass spectrometry methods.