PROTEOMIC ANALYSIS OF EXTINCT SPECIES TO VALIDATE EVOLUTIONARY LINKS

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 研究人员(Asara et.已开始使用质谱仪从已灭绝物种(如霸王龙和乳齿象)的化石中鉴定蛋白质，以提供与现有物种的进化联系。这些最近的研究已经确定了胶原蛋白和一种包含与多种不同现存分类群相匹配的肽序列的血红蛋白。关于这些已鉴定的多肽序列是有效的还是污染的伪迹，存在很大的争议。 我们目前正在分析六个恐龙化石骨骼样本，三个沉积物样本和一个洞穴熊股骨骨骼样本，看看我们可以用我们的样本制备和质谱仪方法检测到什么蛋白质。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Researchers (Asara et. al., Science 2007) have started using mass spectrometry to identify proteins from fossils that have been preserved in extinct species such as T. rex and mastodon to provide evolutionary links with extant species. These recent studies have identified collagen proteins and a hemoglobin protein that contain peptide sequences that match to multiple different extant taxa. There is much controversy on whether these identified peptides sequences are valid or artifacts of contamination. We are currently analyzing six fossilized dinosaur bone samples, three sediment samples and a bone sample from the femur of a cave bear to see what proteins we can detect using our sample preparation and mass spectrometry methods.