NPR2, YEAST HOMOLOG OF THE HUMAN TUMOR SUPPRESSOR NPRL2, IS A TARGET OF GRR1
NPR2, YEAST HOMOLOG OF THE HUMAN TUMOR SUPPRESSOR NPRL2, IS A TARGET OF GRR1
批准号:
8171475
负责人:
CURT WITTENBERG
金额:
$0.24万
依托单位:
依托单位国家:
美国
项目类别:
财政年份:
2010
资助国家:
美国
项目状态:
已结题
起止时间:
2010-09-01 至 2011-08-31
关键词:
AmmoniumCSNK1A1 geneCellsComputer Retrieval of Information on Scientific Projects DatabaseDataF Box DomainFundingGrantGrowthHomologous GeneHumanInstitutionMass Spectrum AnalysisMeiosisNitrogenPhosphoproteinsPhosphorylationPlayProteinsResearchResearch PersonnelResourcesRoleSkp1-Cullin-F-Box ProteinsSourceTechnologyTumor Suppressor ProteinsUnited States National Institutes of HealthUreaYeastsbasecasein kinase Icell growthmulticatalytic endopeptidase complexmutantoverexpressionpermeaseprotein complexubiquitin-protein ligase
中文摘要
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英文摘要
This subproject is one of many research subprojects utilizing the
resources provided by a Center grant funded by NIH/NCRR. The subproject and
investigator (PI) may have received primary funding from another NIH source,
and thus could be represented in other CRISP entries. The institution listed is
for the Center, which is not necessarily the institution for the investigator.
Npr2, a putative "nitrogen permease regulator" and homolog of the human tumor
suppressor NPRL2, was found to interact with Grr1, the F-box component of the SCF(Grr1)
(Skp1-cullin-F-box protein complex containing Grr1) E3 ubiquitin ligase, by mass
spectrometry-based multidimensional protein identification technology. Npr2 has two PEST
sequences and has been previously identified among ubiquitinated proteins. Like other Grr1
targets, Npr2 is a phosphoprotein. Phosphorylated Npr2 accumulates in grr1Delta mutants,
and Npr2 is stabilized in cells with inactivated proteasomes. Phosphorylation and instability
depend upon the type I casein kinases (CK1) Yck1 and Yck2. Overexpression of Npr2 is
detrimental to cells and is lethal in grr1Delta mutants. Npr2 is required for robust growth in
defined medium containing ammonium or urea as a nitrogen source but not for growth on
rich medium. npr2Delta mutants also fail to efficiently complete meiosis. Together, these
data indicate that Npr2 is a phosphorylation-dependent target of the SCF(Grr1) E3 ubiquitin
ligase that plays a role in cell growth on some nitrogen sources.
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