MRI OF INSECT FLIGHT MUSCLE IN A BEETLE

甲虫昆虫飞行肌的 MRI

• 批准号: 8171630
• 负责人: MICHAEL REEDY
• 金额: $ 0.55万
• 依托单位: DUKE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-07-01 至 2011-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8171630
• 关键词: Abdomen; Architecture; Arizona; Books; Chest; Computer Retrieval of Information on Scientific Projects Database; Dissection; Eating; Electron Microscopy; Family; Female; Fiber; Flying body movement; Funding; Gases; Grant; Image; In Situ; Insecta; Institution; Larva; Leg; Length; Life; Magnetic Resonance Imaging; Muscle; Nitrogen; Order Coleoptera; Plant Roots; Research; Research Personnel; Resolution; Resources; Source; Thick; United States National Institutes of Health; Water; Width; flyer; hatching; interest; male

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Body length Body width Body thickness Male 70 mm 20 mm 15 mm Female 70-75 mm 20-22 mm 1) Image 3D internal architecture of flight muscle in thorax (and include abdomen in MRI) of a large beetle known to be a strong flyer (palo verde root borers (Derobrachus geminatus) just collected and fedexed from Tucson, Arizona). The size of the male beetle's body is 70 mm long, 20 mm wide and 15 mm thick. Legs and antennae can be trimmed or removed if not easily tucked in alongside the body of a dead beetle. I want the MRI in order to have an in situ 3D view of the flight muscle undisturbed by dissection. 100 um resolution is ok. 10 um might be interesting if avsilable and 100 um already booked up. Beetles are so heavily sclerotized that gentle flight muscle dissection is very difficult; I have never suceeded with random-catch local NC beetles. Beetles are the last of 4 families of flying insects whose shared type of flight muscle I want to study by electron microscopy and fiber x-ray diffraction. I will asphyxiate a beetle with HCN or nitrogen gas just before starting MRI. Only the larvae eat. The male beetles are not known to eat after hatching and flying about to find and impregnate females. I don't know yet how long they can live on water alone, maybe less than a week.

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 体长体宽体厚 公头70毫米20毫米15毫米 女性70-75毫米20-22毫米 1)一种大型甲虫(刚从亚利桑那州图森市采集到的帕洛韦德钻根虫(Derobrachus Gminatus)，来自亚利桑那州图森市的FedExed)的胸部(MRI中包括腹部)飞行肌肉的3D内部结构图像。这只雄虫的身体长70毫米，宽20毫米，厚15毫米。腿和触角可以被修剪或移除，如果不容易被塞进身体旁边的甲虫身体。我想要核磁共振成像，以便在不受解剖干扰的情况下获得飞行肌肉的原位3D视图。100um分辨率就可以了。10微米可能会很有趣，如果可以的话，100微米已经订完了。甲虫是如此严重的僵化，以至于温和的飞行肌肉解剖是非常困难的；我从来没有成功地随机捕获过当地的NC甲虫。甲虫是飞行昆虫4科中的最后一种，我想用电子显微镜和纤维X射线衍射来研究它们共有的飞行肌肉类型。在开始核磁共振之前，我会用HCN或氮气窒息一只甲虫。只有幼虫才吃东西。雄性甲虫在孵化并四处飞行寻找雌性并使其受孕后，并不会捕食。我还不知道它们光靠水能活多久，也许不到一周。