MICRO-CT IMAGING OF AVASTIN-TREATED MICE WITH LS174TT TUMORS

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目及 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 目的：研究阿瓦斯汀治疗导致血管正常化的假设。我们将使用带有 Isovue 370 的动态显微 CT 来可视化灌注，并使用带有脂质体造影剂的静态显微 CT 来可视化肿瘤的渗透性。 12 只具有存活肿瘤的动物将于 10 年 1 月 5 日从皮埃蒙特研究中心转移到杜克大学。 6 只小鼠将接受阿瓦斯汀治疗，6 只小鼠将作为对照。治疗包括每两周腹膜内注射 5 毫克/公斤的阿瓦斯汀，治疗时间为：从 1 月 7 日星期四开始的星期四和星期一。 第一个成像日为 1 月 8 日星期五。 4个治疗组： 1) 对照脂质体，3 只动物 (CL) 2) 对照灌注，3 只动物 (CP) 3) 处理过的脂质体，3 只动物 (TL) 4) 经处理的灌注，3 只动物 (TP) CL组和TL组每周一通过尾静脉注射脂质体造影剂（0.3ml/25g小鼠），每周一（0小时）和周三（48小时）进行静态显微CT成像。 CP 组和 TP 组将在周五每周使用动态显微 CT 进行一次成像以比较灌注。对于这些研究，我们将使用尾静脉导管以 0.5ml/25g 小鼠的剂量输送 Isovue 370。每次成像后将恢复动物。我们预计未经治疗的小鼠（CL、CP）将在大约 17 天内到达终点，需要将其处死，第 0 天从 1 月 5 日开始。接受治疗的动物（TL、TP）将在约36天内达到终点。因此，对组 (CP,CP) 中的 6 只动物进行成像仅需要 2 周时间。对于未经治疗的动物，成像可持续长达 5 周。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Aim: Study the hypothesis of vascular normalization as a result of Avastin treatment. We will use both dynamic micro-CT with a Isovue 370 to visualize perfusion and static micro-CT with a liposomal contrast agent to visualize permeability in tumors. 12 animals with viable tumors will be transfered from Piedmont Research Center to Duke on 1-5-10. 6 mice will be treated with Avastin and 6 mice will be controls. Treatment involves 5mg/kg of Avastin given IP biweekly, and will take place on: Thursdays and Mondays starting on Thursday January 7th. The first imaging day will be Friday January 8th. 4 treatment groups: 1) Control Liposome, 3 animals (CL) 2) Control Perfusion, 3 animals (CP) 3) Treated Liposome, 3 animals ( TL) 4) Treated Perfusion, 3 animals (TP) The groups CL and TL will be injected via tail vein with liposomal contrast ( 0.3 ml/25g mouse) on Mondays and static micro-CT imaging will take place on Mondays (0 hours) and Wednesdays (48hours) every week. The groups CP and TP will be imaged once per week on Fridays using dynamic micro-CT to compare perfusion. For these stusdies we will use a tail vein catheter to deliver Isovue 370 in a dose of 0.5ml/25 g mouse). Animals will be recovered after each imaging session. We expect that untreated mice (CL, CP) will reach the end point when they will need to be sacrificed in about 17 days with Day 0 starting on January 5th. The treated animals (TL, TP) will reach the endpoint in about 36 days. Therefore, imaging the 6 animals in groups (CP,CP) will take place for only 2 weeks. For untreated animals the imaging could last up to 5 weeks.