STRUCTURAL STUDIES OF THE EXOMER COAT PROTEIN COMPLEX

外显子外壳蛋白复合物的结构研究

• 批准号: 8171519
• 负责人: J Christopher Fromme
• 金额: $ 2.08万
• 依托单位: CORNELL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-07-01 至 2011-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8171519
• 关键词: Binding; Bypass; Capsid Proteins; Carrier Proteins; Cell membrane; Complex; Computer Retrieval of Information on Scientific Projects Database; Data; Data Set; Defect; Eukaryotic Cell; Funding; Grant; Home environment; Institution; Membrane; Membrane Protein Traffic; Mutation; Play; Process; Proteins; Research; Research Personnel; Resources; Role; Secretory Component; Shapes; Source; Structure; System; Testing; United States National Institutes of Health; Vesicle; Yeasts; human disease; protein complex; trafficking; trans-Golgi Network

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. A full third of all gene products are thought to be shuttled through the secretory system of eukaryotic cells. There are numerous human diseases associated with defects in components of the secretory system, or with mutations that disrupt the ability of a protein to interface with the membrane trafficking machinery. Transport of protein ¿¿Scargo¿¿¿ between membrane-bound compartments is a tightly regulated process involving vesicular membrane carriers. A classical membrane vesicle is created via membrane deformation achieved in part by cytosolic ¿¿Scoat¿¿¿ proteins. Coat proteins not only shape membranes, but also play an important role in selecting cargo incorporated into transport carriers. Recent studies have established the existence of a new membrane coat complex responsible for trafficking select cargo from the trans-Golgi network (TGN) to the plasma membrane in yeast, bypassing the endosomal system. We have crystallized a subcomplex of the exomer coat and hope to determine its structure as a step towards understanding how it functions. At this point, our crystals are small and we have not yet tested their diffraction on a home x-ray source, and we believe CHESS beamtime will be required to collect meaningful data. We will use our CHESS beamtime to screen through our various crystal forms and cryo-protectant conditions, and with luck we will be collecting full native datasets.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目和 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 据认为，三分之一的基因产物是通过真核细胞的分泌系统传递的。 许多人类疾病都与分泌系统成分的缺陷有关，或者与破坏蛋白质与膜运输机制相互作用的能力的突变有关。膜结合区室之间蛋白质“Scargo”的运输是一个涉及囊泡膜载体的严格调控过程。 经典的膜囊泡是通过部分由胞质“外套”蛋白实现的膜变形而产生的。 外壳蛋白不仅塑造膜，而且在选择运输载体中的货物方面发挥着重要作用。 最近的研究证实存在一种新的膜涂层复合物，负责将选定的货物从跨高尔基体网络（TGN）绕过内体系统运输到酵母的质膜。 我们已经结晶了外显子外壳的子复合物，并希望确定其结构，作为了解其功能的一步。 目前，我们的晶体很小，我们还没有在家用 X 射线源上测试它们的衍射，我们相信需要 CHESS 波束时间来收集有意义的数据。 我们将使用 CHESS Beamtime 来筛选各种晶体形式和冷冻保护条件，幸运的是，我们将收集完整的本地数据集。