Non-human primate (NHP) Core

非人灵长类 (NHP) 核心

• 批准号: 8198212
• 负责人: NATASA STRBO
• 金额: $ 129.85万
• 依托单位: UNIVERSITY OF MIAMI SCHOOL OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-09-01 至 2014-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8198212
• 关键词: Animals; Antibodies; Arteries; Autopsy; Biological Markers; Blood specimen; Cervix Uteri; Diagnostic; Disease; Dissection; Euthanasia; Evaluation; Figs - dietary; Fixatives; Formaldehyde; Freezing; Fresh Tissue; Gene Expression; HIV; HIV vaccine; Health Status; Hematoxylin and Eosin Staining Method; Human; Human Papillomavirus; Human Resources; Immune response; Immunohistochemistry; In Situ Hybridization; Infection; Institutes; Laboratories; Laboratory Animals; Lead; Lesion; Ligaments; Macaca mulatta; Modeling; Molecular; Nanotechnology; Operative Surgical Procedures; Organ; Paraffin; Parametrial; Pathologist; Pathology; Preparation; Procedures; Process; Protocols documentation; Rectum; Research; Research Personnel; Research Project Grants; SIV; SIV Vaccines; Sampling; Shipping; Ships; Specimen; Spleen; Staining method; Stains; Sterility; Structure of internal iliac vein; Techniques; Time; Tissue Banks; Tissues; Vaccines; Vagina; Veterinarians; design; experience; external iliac vein; jejunum; laser capture microdissection; lymph nodes; macromolecule; nonhuman primate; novel strategies; novel vaccines; tissue fixing; vaccine development

The Pathology Core has a unique team of investigators with the professional expertise to assist with the design and implementation of the unique vaccine strategies proposed. The Core Director, Dr. Altman, is a veterinary pathologist and laboratory animal veterinarian. He will coordinate tissue collection, presevation, sample preparation, and distribution. Additional, he will assist in the intrepatation of tissue changes and biomarkers. The Co-Director, Dr. Nadji, is a surgical pathologist with expertise in diagnostic and predictive biomarkers. He has developed unique procedures which will be extremely important in identifing the molecular and cellular mechanisms involved in the immune responses. The techniques proposed including immunohistochemistry and in situ hybridization are highly sensitive in identification of cellular changes. The procedures utilized will preserve macromolecules in tissue traditionally achieved by immediate freezing. The Research lnvestigator, Dr. Datar, is the Co-Director of the UM Biomedical Nanotechnology Institute and an experienced molecular biologist with many years of experience in gene expression analysis of fresh and archival tissues. He will lead the Laser Capture Microdissection procedues which are critical to the research projects in this proposal. Lay Summary: This project is targeted to use two novel vaccine strategies in a Rhesus monkey model of SIV infection to understand the molecular and cellular mechanisms that provide protection against this disease. State-of-the art-techniques and procedures will be utilized to characterize changes at critical time points after infection. The information obtained will help to develop an effective HIV vaccine for humans.

病理学核心有一个独特的研究团队，具有专业知识，以协助 设计和实施所提出的独特疫苗战略。核心主任Altman博士是一位 兽医病理学家和实验动物兽医。他将协调组织收集，保存， 样品制备和分发。此外，他将协助组织变化的治疗， 生物标志物。联合主任Nadji博士是一名外科病理学家，在诊断和预测方面具有专长。 生物标志物。他开发了独特的程序，这将是非常重要的，在确定 参与免疫反应的分子和细胞机制。提出的技术包括 免疫组织化学和原位杂交在鉴定细胞变化中是高度敏感的。的 所使用的程序将保存传统上通过立即冷冻实现的组织中的大分子。的 研究调查员Datar博士是UM生物医学纳米技术研究所的联合主任， 经验丰富的分子生物学家，具有多年的基因表达分析经验， 存档组织。他将领导对研究至关重要的激光捕获显微切割程序 项目在这个提案中。 概述：该项目旨在在恒河猴模型中使用两种新的疫苗策略， SIV感染，以了解提供保护的分子和细胞机制， 疾病最先进的技术和程序将被用来描述关键时刻的变化 感染后。所获得的信息将有助于为人类开发有效的艾滋病毒疫苗。