Zero Net Charge Polymer Brush Surfaces Highly Resistant to Protein Adsorption
零净电荷聚合物刷表面高度抵抗蛋白质吸附
基本信息
- 批准号:8263028
- 负责人:
- 金额:$ 5.39万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2011
- 资助国家:美国
- 起止时间:2011-05-01 至 2013-04-30
- 项目状态:已结题
- 来源:
- 关键词:AdsorptionAdvanced DevelopmentBackBehaviorBiocompatibleBiocompatible MaterialsBiosensorBloodBody FluidsCaliberCardiovascular systemCell AdhesionCell Culture TechniquesCell-Matrix JunctionCellsCharacteristicsChargeChemicalsCuesDevicesDiagnosticElectrostaticsEngineeringEnsureEquilibriumEventFutureHealthInfectionInflammationInflammatory ResponseInterventionInvestigationKnowledgeLeadLengthLiteratureMedicalMedical DeviceMedical StudentsModificationMolecularMolecular StructureOutcomeOutcome StudyPatientsPerformancePlaguePlasma ProteinsPolymersPropertyProsthesisProteinsReportingResearchResearch PersonnelResearch TrainingResistanceS PhaseScientistSeriesSolidStructure-Activity RelationshipSurfaceSystemTechniquesTechnologyTherapeuticThrombosisTissue EngineeringVisionadverse outcomecostdensitydesignimplantable deviceimplanted sensorimprovedmicroorganismmonomermultidisciplinarynovelpeptidomimeticsphysical propertyphysical separationpolymerizationpreventpublic health relevanceresearch studysensor
项目摘要
DESCRIPTION (provided by applicant): The creation of durable, functional and biocompatible implants and sensors is key to improved patient outcomes and lower medical costs. Surface fouling of body-fluid-contacting devices by non-specific protein adsorption and cell attachment, and the consequent surface-induced thrombotic and inflammatory responses, degrade device performance and lead to adverse outcomes that require medical intervention. The extensive literature on surface modifications aimed at preventing bio-fouling highlights both the broad potential and the current limitations of this strategy for improving medical device and health outcomes. Recent reports have demonstrated that certain zwitterionic and ampholytic polymers are effective at inhibiting biofouling. However, a mechanistic understanding of this behavior is lacking. We hypothesize that controlling the spatial separation of oppositely charged groups within a certain length-scale smaller than the size of proteins, while maintaining the zero net charge in a polymer brush, imparts the brush surface with resistance to protein adsorption. Accordingly, this project aims to design and synthesize zero net charge polymer brushes with a range of separation between oppositely charged groups, evaluate their resistance to protein adsorption and cell adhesion, and analyze the pertinent structure-function relationships. The systematic study of the charge separation will be enabled by the solid phase synthesis of peptidomimetic polymers. This technique allows absolute control over monomer sequence, brush length and thus charge separation. This research training will be aided by the multidisciplinary profile of the investigator's research group, consisting of medical students and scientists in cell, chemical and materials engineering. The outcomes of this study will include identification of novel anti-fouling brush surfaces applicable to therapeutic and diagnostic devices, and fundamental knowledge of the configurational requirements for imparting anti-fouling properties to (pseudo)zwitterionic materials. The investigation of polymer systems possessing molecular level, sequence-specific chemical and spatial cues will advance the development of biomaterials that elicit desirable biorecognition properties.
PUBLIC HEALTH RELEVANCE: This experimental study will systematically investigate the molecular structure required to confer anti-fouling properties to polymers possessing a balanced number of electrostatic charges. Coating biomedical devices with such anti-fouling polymers would prevent unwanted fouling by biomolecules and cells, which would improve device performance and patient outcomes, and lower medical costs.
描述(由申请人提供):创造耐用、功能和生物兼容的植入物和传感器是改善患者预后和降低医疗成本的关键。体液接触设备的表面由非特异性蛋白质吸附和细胞附着造成的污垢,以及随之而来的表面诱导的血栓和炎症反应,降低了设备的性能,并导致需要医疗干预的不良后果。关于防止生物污染的表面改性的大量文献突出了这一战略在改善医疗器械和健康结果方面的广泛潜力和当前的局限性。最近的报道表明,某些两性离子聚合物和两性聚合物在抑制生物污垢方面是有效的。然而,对这种行为缺乏机械性的理解。我们假设,在小于蛋白质尺寸的一定长度-尺度内控制相反电荷基团的空间分离,同时保持聚合物刷子中的零净电荷,赋予刷子表面对蛋白质吸附的阻力。因此,本项目旨在设计和合成具有相反电荷基团之间分离范围的零净电荷聚合物刷子,评估其对蛋白质吸附和细胞黏附的抗性,并分析相关的结构-功能关系。模拟多肽聚合物的固相合成将使电荷分离的系统研究成为可能。这项技术允许绝对控制单体顺序、电刷长度,从而实现电荷分离。这项研究培训将得到研究人员研究小组的多学科概况的帮助,研究小组由细胞、化学和材料工程方面的医学生和科学家组成。这项研究的成果将包括识别适用于治疗和诊断设备的新型防污刷表面,以及赋予(伪)两性离子材料防污性能的结构要求的基本知识。对具有分子水平、特定序列的化学和空间线索的聚合物体系的研究将推动生物材料的发展,使其具有理想的生物识别性能。
与公众健康相关:这项实验研究将系统地研究赋予具有平衡数量静电电荷的聚合物防污性能所需的分子结构。用这种防污染聚合物涂覆生物医疗设备将防止生物分子和细胞产生不必要的污染,这将改善设备性能和患者预后,并降低医疗成本。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
数据更新时间:{{ journalArticles.updateTime }}
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
数据更新时间:{{ journalArticles.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ monograph.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ sciAawards.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ conferencePapers.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ patent.updateTime }}
K. H. Aaron Lau其他文献
K. H. Aaron Lau的其他文献
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
{{ truncateString('K. H. Aaron Lau', 18)}}的其他基金
Zero Net Charge Polymer Brush Surfaces Highly Resistant to Protein Adsorption
零净电荷聚合物刷表面高度抵抗蛋白质吸附
- 批准号:
8126946 - 财政年份:2011
- 资助金额:
$ 5.39万 - 项目类别:
相似海外基金
ADVANCED DEVELOPMENT OF LQ A LIPOSOME-BASED SAPONIN-CONTAINING ADJUVANT FOR USE IN PANSARBECOVIRUS VACCINES
用于 Pansarbecovirus 疫苗的 LQ A 脂质体含皂苷佐剂的先进开发
- 批准号:
10935820 - 财政年份:2023
- 资助金额:
$ 5.39万 - 项目类别:
ADVANCED DEVELOPMENT OF BBT-059 AS A RADIATION MEDICAL COUNTERMEASURE FOR DOSING UP TO 48H POST EXPOSURE"
BBT-059 的先进开发,作为辐射医学对策,可在暴露后 48 小时内进行给药”
- 批准号:
10932514 - 财政年份:2023
- 资助金额:
$ 5.39万 - 项目类别:
Advanced Development of a Combined Shigella-ETEC Vaccine
志贺氏菌-ETEC 联合疫苗的先进开发
- 批准号:
10704845 - 财政年份:2023
- 资助金额:
$ 5.39万 - 项目类别:
Advanced development of composite gene delivery and CAR engineering systems
复合基因递送和CAR工程系统的先进开发
- 批准号:
10709085 - 财政年份:2023
- 资助金额:
$ 5.39万 - 项目类别:
Advanced development and validation of an in vitro platform to phenotype brain metastatic tumor cells using artificial intelligence
使用人工智能对脑转移肿瘤细胞进行表型分析的体外平台的高级开发和验证
- 批准号:
10409385 - 财政年份:2022
- 资助金额:
$ 5.39万 - 项目类别:
ADVANCED DEVELOPMENT OF A VACCINE FOR PANDEMIC AND PRE-EMERGENT CORONAVIRUSES
针对大流行和突发冠状病毒的疫苗的高级开发
- 批准号:
10710595 - 财政年份:2022
- 资助金额:
$ 5.39万 - 项目类别:
Advanced development and validation of an in vitro platform to phenotype brain metastatic tumor cells using artificial intelligence
使用人工智能对脑转移肿瘤细胞进行表型分析的体外平台的高级开发和验证
- 批准号:
10630975 - 财政年份:2022
- 资助金额:
$ 5.39万 - 项目类别:
ADVANCED DEVELOPMENT OF A VACCINE CANDIDATE FOR STAPHYLOCOCCUS AUREUS INFECTION
金黄色葡萄球菌感染候选疫苗的高级开发
- 批准号:
10710588 - 财政年份:2022
- 资助金额:
$ 5.39万 - 项目类别:
ADVANCED DEVELOPMENT OF A VACCINE FOR PANDEMIC AND PRE-EMERGENT CORONAVIRUSES
针对大流行和突发冠状病毒的疫苗的高级开发
- 批准号:
10788051 - 财政年份:2022
- 资助金额:
$ 5.39万 - 项目类别: