STRUCTURAL BIOLOGY
结构生物学
基本信息
- 批准号:8378393
- 负责人:
- 金额:$ 22.57万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2012
- 资助国家:美国
- 起止时间:2012-05-21 至 2015-04-30
- 项目状态:已结题
- 来源:
- 关键词:BindingBiologicalBiologyBudgetsCalorimetryCancer CenterCancer Center Support GrantCell NucleusCellsChemicalsComputer softwareCrystallizationCrystallographyDNAData CollectionDevelopmentEquipmentFundingFutureHuman ResourcesImageIndividualInsectaInstitutesInvestmentsKineticsLaboratoriesLigandsMammalian CellMapsMeasurementMeasuresMechanicsMolecularNatureOpticsPaperPeer ReviewPlayProcessProductionProtein DynamicsProteinsPublicationsRNAResearchResearch PersonnelResolutionResource SharingResourcesRobotRoentgen RaysRoleSamplingScanningScienceScientistScreening procedureServicesShapesSiteSourceStructureSystemTechniquesTechnologyThermodynamicsTitrationsTrainingWalkingWorkanticancer researchbasecell behaviorcell motilitydetectordrug discoveryexperienceinhibitor/antagonistinstrumentinstrumentationlarge scale productionmacromoleculemolecular sizeoperationprogramsprotein expressionprotein purificationsmall moleculestopped-flow fluorescencestructural biologythree dimensional structure
项目摘要
Ttie Structural Biology Shared Resource brings togethier Crystallograpfiy, NMR, and Protein Production &
Analysis into a single facility, with Dr. Liddington as its Scientific Director and two Facility Directors/Managers
who oversee day-to-day operations. This merger has brought the two complementary techniques for
determining 3-dimensional structures of biological macromolecules at atomic resolution together with
facilities for the large-scale production and biophysical characterization of proteins, technologies that are not
available in individual Cancer Center laboratories owing to their expense and complexity. These
developments have created a seamless Resource that is transforming the ability of scientists with little prior
experience in structural biology to incorporate structural and structure-function studies as well as chemical
biology into their research programs. The expanded facility has served the needs of more than 40 Cancer
Center investigators over the last few years. The Institute has provided major equipment upgrades in
crystallography, including a high brilliance generator fully equipped for data collection on two ports, together
with a crystallization robot and storage/imaging "hotels"; a new cryoprobe for the 600 MHz NMR instrument
and a new "walk-up" 400 MHz instrument optimized for rapidly tuning to different nuclei and high-throughput
sample-changing; and a complete laboratory suite for large-scale protein expression and purification in
bacterial, insect and mammalian cells, together with state-of-the-art instrumentation for biophysical analysis,
including an Analytical Ultracentrifuge, Differential Scanning and Isothermal Titration Calorimetry and
stopped-flow fluorescence spectrometer for measurement of molecular size, shape, folded integrity, and
thermodynamic and kinetic binding constants. The Resource directly supported Cancer Center investigator's
work in the past funding period that led to the publication of at least 83 peer-reviewed papers, including 5 in
Nature, Cell or Science, as well as more than 40 review articles on various aspects of structural biology and
structure-function studies. Future priorities include the full integration of the crystallization robot facility into
drug discovery efforts; additional instrumentation to measure binding kinetics of protein-small molecule
interactions, and to measure mechanical forces within and between molecules and their interplay with
chemical forces in processes such as cell migration; and further upgrades in eukaryotic protein expression
and purification systems. Overall, $139,456 in CCSG support is requested in the first year, representing 12%
of the total projected Structural Biology operating budget.
结构生物学共享资源汇集了晶体学,核磁共振和蛋白质生产&
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
数据更新时间:{{ journalArticles.updateTime }}
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
数据更新时间:{{ journalArticles.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ monograph.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ sciAawards.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ conferencePapers.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ patent.updateTime }}
ROBERT Colin LIDDINGTON其他文献
ROBERT Colin LIDDINGTON的其他文献
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
{{ truncateString('ROBERT Colin LIDDINGTON', 18)}}的其他基金
Interrogating the role of complement MAC in the pathogenesis of age-related macular degeneration: Structure-enhanced discovery of probes and leads for novel therapies
探究补体 MAC 在年龄相关性黄斑变性发病机制中的作用:结构增强的探针和新疗法先导化合物的发现
- 批准号:
9010453 - 财政年份:2016
- 资助金额:
$ 22.57万 - 项目类别:
Interrogating the role of complement MAC in the pathogenesis of age-related macular degeneration: Structure-enhanced discovery of probes and leads for novel therapies
探究补体 MAC 在年龄相关性黄斑变性发病机制中的作用:结构增强的探针和新疗法先导化合物的发现
- 批准号:
9206174 - 财政年份:2016
- 资助金额:
$ 22.57万 - 项目类别:
Assembly, dynamics and evolution of cell-cell and cell-matrix adhesions
细胞-细胞和细胞-基质粘附的组装、动力学和进化
- 批准号:
8438592 - 财政年份:2010
- 资助金额:
$ 22.57万 - 项目类别:
Assembly, dynamics and evolution of cell-cell and cell-matrix adhesions
细胞-细胞和细胞-基质粘附的组装、动力学和进化
- 批准号:
8814966 - 财政年份:2010
- 资助金额:
$ 22.57万 - 项目类别:
Assembly, dynamics and evolution of cell-cell and cell-matrix adhesions
细胞-细胞和细胞-基质粘附的组装、动力学和进化
- 批准号:
8307835 - 财政年份:2010
- 资助金额:
$ 22.57万 - 项目类别:
Assembly, dynamics and evolution of cell-cell and cell-matrix adhesions
细胞-细胞和细胞-基质粘附的组装、动力学和进化
- 批准号:
8513360 - 财政年份:2010
- 资助金额:
$ 22.57万 - 项目类别:
Assembly, dynamics and evolution of cell-cell and cell-matrix adhesions
细胞-细胞和细胞-基质粘附的组装、动力学和进化
- 批准号:
8150411 - 财政年份:2010
- 资助金额:
$ 22.57万 - 项目类别:
CRYSTALLOGRAPHIC STUDIES OF CELL MIGRATION AND HOST-PATHOGEN INTERACTIONS
细胞迁移和宿主-病原体相互作用的晶体学研究
- 批准号:
8169953 - 财政年份:2010
- 资助金额:
$ 22.57万 - 项目类别:
Assembly, dynamics and evolution of cell-cell and cell-matrix adhesions
细胞-细胞和细胞-基质粘附的组装、动力学和进化
- 批准号:
7982331 - 财政年份:2010
- 资助金额:
$ 22.57万 - 项目类别:
相似海外基金
Elucidating the molecular basis and expanding the biological applications of the glycosyltransferases using biochemical and structural biology approaches
利用生化和结构生物学方法阐明糖基转移酶的分子基础并扩展其生物学应用
- 批准号:
23K14138 - 财政年份:2023
- 资助金额:
$ 22.57万 - 项目类别:
Grant-in-Aid for Early-Career Scientists
Upsampling of low-resolution/large-volume 3D tomographic images using generative adversarial neural networks applied to biological anthropology, medical imaging, and evolutionary biology
使用应用于生物人类学、医学成像和进化生物学的生成对抗神经网络对低分辨率/大容量 3D 断层扫描图像进行上采样
- 批准号:
571519-2021 - 财政年份:2022
- 资助金额:
$ 22.57万 - 项目类别:
Alliance Grants
The biology of Ciceribacter spp. and their adaptations as biological chassis for engineered nitrogen fixation
西塞里杆菌属的生物学。
- 批准号:
2735213 - 财政年份:2022
- 资助金额:
$ 22.57万 - 项目类别:
Studentship
NSF Postdoctoral Fellowship in Biology: Symbiosis as a Means of Survival for Biological Soil Crust Microbes
NSF 生物学博士后奖学金:共生作为生物土壤结皮微生物的生存手段
- 批准号:
2209217 - 财政年份:2022
- 资助金额:
$ 22.57万 - 项目类别:
Fellowship Award
Conference: 2023 Stochastic Physics in Biology: Bridging Stochastic Physical Theories with Biological Experiments
会议:2023 年生物学中的随机物理学:将随机物理理论与生物实验联系起来
- 批准号:
2242530 - 财政年份:2022
- 资助金额:
$ 22.57万 - 项目类别:
Standard Grant
From Big Biological Data to Tangible Insights: Designing tangible and multi-display interactions to support data analysis and model building in the biology domain
从生物大数据到有形洞察:设计有形和多显示交互以支持生物学领域的数据分析和模型构建
- 批准号:
RGPIN-2021-03987 - 财政年份:2022
- 资助金额:
$ 22.57万 - 项目类别:
Discovery Grants Program - Individual
Engineering of next-generation synthetic biology tools for biological applications
用于生物应用的下一代合成生物学工具的工程
- 批准号:
RGPIN-2019-07002 - 财政年份:2022
- 资助金额:
$ 22.57万 - 项目类别:
Discovery Grants Program - Individual
BEORHN: Biological Enzymatic Oxidation of Reactive Hydroxylamine in Nitrification via Combined Structural Biology and Molecular Simulation
BEORHN:通过结合结构生物学和分子模拟对硝化反应中的活性羟胺进行生物酶氧化
- 批准号:
BB/V01577X/1 - 财政年份:2022
- 资助金额:
$ 22.57万 - 项目类别:
Research Grant
NSF Postdoctoral Fellowship in Biology FY 2020: Integrating biological collections and observational data sources to estimate long-term butterfly population trends
2020 财年 NSF 生物学博士后奖学金:整合生物收藏和观测数据源来估计蝴蝶种群的长期趋势
- 批准号:
2010698 - 财政年份:2021
- 资助金额:
$ 22.57万 - 项目类别:
Fellowship Award
Engineering of next-generation synthetic biology tools for biological applications
用于生物应用的下一代合成生物学工具的工程
- 批准号:
RGPIN-2019-07002 - 财政年份:2021
- 资助金额:
$ 22.57万 - 项目类别:
Discovery Grants Program - Individual