Animal Core

项目总结（见说明）： 核心实验室将由一位经验丰富的博士后研究员指导。赵金存博士，在核心领导人帕尔曼博士的指导下。核心将主要位于配备组织培养和动物工作的BSL 3实验室。核心工作人员在病毒学和细胞培养技术方面经验丰富。动物核心有几个功能，这是成功的政治人物授予的关键。它将繁殖和滴度非重组和重组小鼠适应和Urbani菌株， SARS-CoV并将这些病毒感染小鼠。它将监测小鼠的临床疾病和体重减轻，并将收获组织。核心将制备用于组织学和免疫组织化学分析的样本，并将从感染组织中制备RNA和蛋白质。核心将分析小鼠的抗体和T细胞反应。该中心还将负责确保与动物一起工作的协议， 在BSL 3实验室条件下工作是最新的。虽然核心将教授项目成员在BSL 3条件下分析小鼠的重要方法，但它将主要负责执行大多数这些分析。核心将提供试剂，并有效地进行常见的测定，从而标准化结果并增强协同作用。此外，所有项目都将与 核心，从而促进PPG成员之间的互动。通过提供这些服务，核心将使项目研究人员能够专注于与SARS-CoV发病机制以及抗病毒疗法和疫苗开发相关的问题。

PROJECT SUMMARY (See instructions): The Core Laboratory will be directed by an experienced postdoctoral fellow. Dr. Jincun Zhao, under the guidance of the Core Leader, Dr. Perlman. The Core will be primarily based in a BSL3 laboratory that is equipped for tissue culture and animal work. Personnel working in the Core are experienced in virological and cell culture techniques. The Animal Core has several functions that are critical for success of this POl grant. It will propagate and titer nonrecombinant and recombinant mouse-adapted and Urbani strains of SARS-CoV and will infect mice with these viruses. It will monitor mice for clinical disease and weight loss and will harvest tissue. The Core will prepare samples for histological and immunohistochemical analysis and will prepare RNA and protein from infected tissue. The Core will analyze mice for antibody and T cell responses. The Core will also be responsible for ensuring that protocols for working with animals and for working under BSL3 laboratory conditions are up-to-date. While the Core will teach members of the Project methods important for analysis of mice under BSL3 conditions, it will primarily be responsible for performing most of these analyses. The Core will provide reagents and perform common assays efficiently, thereby standardizing results and enhancing synergistic interactions. Additionally, all projects will interact with the Core, thereby facilitating interactions between members of the PPG. By providng these services, the Core will allow Program investigators to focus on issues related to SARS-CoV pathogenesis and to the development of anti-viral therapies and vaccines.