Animal Core

项目总结(见说明)： 核心实验室将由一位经验丰富的博士后研究员领导。赵金村博士，在核心领导帕尔曼博士的指导下。核心将主要以BSL3实验室为基础，该实验室配备有组织培养和动物工作。在核心中心工作的人员在病毒学和细胞培养技术方面经验丰富。动物核心有几项功能对这笔POL赠款的成功至关重要。它将繁殖和滴度非重组和重组小鼠适应和Urbani菌株 SARS冠状病毒，并将感染这些病毒的老鼠。它将监测小鼠的临床疾病和体重减轻，并将收集组织。该中心将准备用于组织学和免疫组织化学分析的样本，并将从感染组织中制备RNA和蛋白质。Core将分析小鼠的抗体和T细胞反应。核心还将负责确保与动物一起工作的协议和 在BSL3实验室条件下工作是最新的。虽然核心将教授项目成员在BSL3条件下分析MICE的重要方法，但它将主要负责执行大多数这些分析。核心中心将提供试剂并有效地执行常见的分析，从而使结果标准化并增强协同作用。此外，所有项目都将与 核心，从而促进PPG成员之间的互动。通过提供这些服务，核心中心将使项目调查人员能够专注于与SARS-CoV发病机制以及抗病毒疗法和疫苗的开发有关的问题。

PROJECT SUMMARY (See instructions): The Core Laboratory will be directed by an experienced postdoctoral fellow. Dr. Jincun Zhao, under the guidance of the Core Leader, Dr. Perlman. The Core will be primarily based in a BSL3 laboratory that is equipped for tissue culture and animal work. Personnel working in the Core are experienced in virological and cell culture techniques. The Animal Core has several functions that are critical for success of this POl grant. It will propagate and titer nonrecombinant and recombinant mouse-adapted and Urbani strains of SARS-CoV and will infect mice with these viruses. It will monitor mice for clinical disease and weight loss and will harvest tissue. The Core will prepare samples for histological and immunohistochemical analysis and will prepare RNA and protein from infected tissue. The Core will analyze mice for antibody and T cell responses. The Core will also be responsible for ensuring that protocols for working with animals and for working under BSL3 laboratory conditions are up-to-date. While the Core will teach members of the Project methods important for analysis of mice under BSL3 conditions, it will primarily be responsible for performing most of these analyses. The Core will provide reagents and perform common assays efficiently, thereby standardizing results and enhancing synergistic interactions. Additionally, all projects will interact with the Core, thereby facilitating interactions between members of the PPG. By providng these services, the Core will allow Program investigators to focus on issues related to SARS-CoV pathogenesis and to the development of anti-viral therapies and vaccines.