HIGH-THROUGHPUT GENE EXPRESSION

对于单个项目，将提供特定细胞、动物和临床表型的基因表达分析。重要的是，该核心实验室将开发定制的靶向基因表达和miRNA阵列，这些阵列将提供检查和直接比较涉及内皮功能，线粒体生物发生和功能，活性氧，脂肪细胞，巨噬细胞和缺氧/血管生成的相关基因集的能力。基因表达核心以前曾与Gokce博士合作或发表过 他已经开始与科恩和沃尔什博士一起研究miRNA和高通量基因表达。 基因表达核心实验室是一个成熟的设施，具有使用计算机程序的自动机器人移液，自动RNA分离，储存和定制芯片容量。该实验室已经参与了来自各种临床项目的10，000多个受试者的基因表达，miRNA和蛋白质样本的高通量分析。该实验室还协助研究人员使用小鼠和细胞培养模型进行小体积组织的基因表达分析。 重要的是，将开发一组已建立的基因和miRNA，将在研究中进行比较，以确定特定炎症状态和代谢疾病之间的机制重叠以及单个细胞类型在内皮和血管疾病中的作用（见表1）。

For the individual projects, gene expression analyses will be provided for specific cellular, animal, and clinical phenotypes. Importantly, this core lab will develop custom targeted gene expression and miRNA arrays that will provide the capacity to examine and directly compare related gene sets involved in endothelial function, mitochondrial biogenesis and function, reactive oxygen species, adipocytes, macrophages and hypoxia/angiogenesis. The Gene Expression Core has previously collaborated or published with Drs. Gokce and Vita and has begun studying miRNA and high-throughput gene expression with Drs. Cohen and Walsh. The Gene Expression Core Laboratory is an established facility with automated robotic pipeting using computerized programs, automated RNA isolation, storage, and custom chip capacity. The Laboratory is already involved in high-throughput analysis of over 10,000 subjects' gene expression, miRNA, and protein samples from various clinical projects. The laboratory has also assisted investigators in gene expression analysis from small volume tissue using murine and cell culture models. Importantly, there will be developed an established panel of genes and miRNA that will be compared across studies to determine the mechanistic overlap between specific inflammatory states and metabolic diseases and the role individual cell types play in endothelial and vascular disease (see Table 1).