Cell Culture Resource Core

核心B是原代内皮细胞培养和腺病毒的中心资源，可用于每个 四个项目。这一核心设施将为以下方面提供服务：1)初级 人肺微血管内皮细胞；2)原代肺的分离、鉴定和培养 小鼠来源的微血管内皮细胞，包括不同基因小鼠模型的微血管内皮细胞和3) 重组腺病毒的扩增和纯化，用于以下项目的实验 在下面的核心描述中被取消。人和小鼠肺内皮细胞培养对 完成所有四个项目中提出的研究。具体地说，核心B对于提供 从不同的遗传小鼠模型中分离出内皮细胞；例如，fikGPF*‘“转基因和Cav-1，eNOS， AKT-1、Src、NOX2和PAR-1基因敲除小鼠

Core B is a central resource for primary endothelial cell cultures and adenoviruses available for each of the four projects. This core facility will provide services for the following: 1) characterization and culture of primary human lung microvascular endothelial cells; 2) isolation, characterization and culture of primary lung microvascular endothelial cells of mouse origin including those of different genetic mouse models and 3) amplification and purification of recombinant adenoviruses for the experiments proposed in the Projects as outiined in the Core description below. Human and mouse lung endothelial cell cultures are pivotal for the accomplishment of studies proposed in all four Projects. Specifically, Core B is critical for providing endothelial cells isolated from different genetic mouse models; e.g.,fiklGPF*'"transgenic and Cav-1, eNOS, AKT-1, Src, Nox2 and PAR-1 knockout mice for