Mesodissection Device

细血管解剖装置

• 批准号: 8253130
• 负责人: Nils B Adey
• 金额: $ 21.3万
• 依托单位: AVANSCI BIO, LLC
• 依托单位国家: 美国
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-03-01 至 2012-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8253130
• 关键词: Area; Aspirate substance; Biological Assay; Biological Models; Cancer Diagnostics; Cell physiology; Cells; Clinical Research; DNA; DNA Fingerprinting; Development; Devices; Diabetes Mellitus; Discipline; Disease; Dissection; Effectiveness; Elements; Excision; Feedback; Genomics; Glass; Goals; Heart Diseases; Height; Histocompatibility Testing; Histopathology; Image; Investments; Laboratories; Licensing; Liquid substance; Malignant Neoplasms; Manuals; Marketing; Mass Spectrum Analysis; Measures; Mechanics; Methods; Microdissection; Microscopic; Molecular; Motor; Movement; Normal Cell; Nucleic Acids; Optics; Performance; Phase; Positioning Attribute; Procedures; Process; Productivity; Recovery; Relative (related person); Research; Research Personnel; Resolution; Reverse Transcriptase Polymerase Chain Reaction; Sampling; Selection Bias; Slide; Specificity; Speed; Staging; System; Techniques; Technology; Testing; Thick; Time; Tissues; Universities; Utah; Vacuum; Validation; Work; anticancer research; base; biological research; commercialization; cost; design; design and construction; digital; digital imaging; expectation; improved; instrument; interest; laser capture microdissection; new technology; pressure; prototype; sample fixation

DESCRIPTION (provided by applicant): Mesodissection Device Microscopic analysis of tissue mounted on glass slides is commonly used to study cancer, nonneoplastic entities such as heart disease and diabetes, and normal cell function. At a growing rate, researchers are utilizing various micro and macro tissue dissection techniques to recover specific areas of interest from a slide mounted tissue section for subsequent molecular testing including real time RTPCR, microarrays, sequencing, and mass spectrometry. The primary goal of the proposed Phase I project is to develop a manufacturing prototype of an inexpensive, manually controlled, dissection instrument for slide mounted tissue sections. This device will be based on an existing lab technology coinvented and exclusively licensed from the University of Utah's ARUP Laboratories. The subsequent commercial instrument would fill the need in the biological research market for a mesodissection (100,m resolution) instrument with the low cost, ease of use, and recovery efficiency of manual dissection techniques, but with far better precision. This device will include xy positioning, integrated optics, and multiple choices of specialized tissue removal and recovery tips. The manufacturing prototype will be tested for resolution, efficiency, and accuracy of tissue recovery. Resolution is defined as the difference in ,m between the circumference of the actual post dissection area and that of the originally indicated area for removal. Efficiency is defined as the percentage of tissue recovered from an indicated area measured by DNA quantification. Accuracy is defined as the percentage of desired DNA divided by the total DNA recovered and will be determined by DNA typing. Validation strategies are defined based on a model system of tissue type and slide type. By quantifying the resolution, efficiency and accuracy of DNA recovery in Phase I on prototype instruments we will demonstrate feasibility for Phase II development and commercialization. PUBLIC HEALTH RELEVANCE: Mesodissection Device The Mesodissection Device will provide a highly affordable system for dissection of tissue sections from glass slides. This device will provide 100m dissection resolution at an order of magnitude lower investment than that required for laser capture microdissection, enabling much broader researcher access to the new technology. The technology will promote productivity and quality of results for researchers working in the fields of histopathology and cancer diagnostics, non!cancer research including heart disease and diabetes, and areas outside of disease research including cell development and cellular function.

描述（由申请人提供）：中间解剖装置对载玻片上的组织进行显微镜分析通常用于研究癌症、非肿瘤实体（如心脏病和糖尿病）以及正常细胞功能。研究人员正以越来越快的速度利用各种微观和宏观组织解剖技术从载玻片上的组织切片中恢复感兴趣的特定区域，用于随后的分子检测，包括真实的时间RTPCR、微阵列、测序和质谱。拟议的第一阶段项目的主要目标是开发一种廉价、手动控制的解剖仪器的制造原型，用于载玻片安装的组织切片。该设备将基于现有的实验室技术，该技术由犹他州大学的ARUP实验室共同发明并独家授权。随后的商业仪器将满足生物研究市场对中间解剖（100 μ m分辨率）仪器的需求，其具有手动解剖技术的低成本、易用性和恢复效率，但具有更好的精度。该器械将包括xy定位、集成光学器件以及多种选择的专用组织切除和恢复尖端。将对制造原型进行组织回收的分辨率、效率和准确度测试。分辨率定义为实际切割后区域的周长与最初指定切除区域的周长之间的差异，m。效率定义为通过DNA定量测量的从指定区域回收的组织的百分比。准确度定义为所需DNA的百分比除以回收的总DNA，并将通过DNA分型确定。根据组织类型和载玻片类型的模型系统定义确认策略。通过在原型仪器上量化第一阶段DNA回收的分辨率、效率和准确性，我们将证明第二阶段开发和商业化的可行性。 公共卫生相关性：中间分离装置中间分离装置将提供一种非常经济实惠的系统，用于从载玻片上分离组织切片。该设备将提供100米的解剖分辨率，比激光捕获显微解剖所需的投资低一个数量级，使更广泛的研究人员能够获得新技术。该技术将提高组织病理学和癌症诊断领域研究人员的工作效率和结果质量。癌症研究，包括心脏病和糖尿病，以及疾病研究以外的领域，包括细胞发育和细胞功能。