DEVELOPING A NOVEL SET OF DIET PATTERN BIOMARKERS

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 这项研究试图开发和验证一套新的饮食生物标记物，基于食物之间自然发生的同位素差异。同位素差异可以区分来自海洋和陆地来源的食物，生长在高纬度和低纬度的食物，以及以玉米或甘蔗为基础的食物。因为爱斯基摩人依赖自给自足的食物和美国市场上的食物，所以他们是测试这些摄入量的生物标志物的理想人群。首先，我们将通过收集和分析常用食物的多个同位素特征，来测试关键食物的同位素特征是否可以用来定义与营养相关的食物组。其次，我们将招募100人参加年龄和性别分层设计，并比较重复饮食记录和红细胞、头发和指甲的同位素特征所测量的摄入量。最后，我们将测试头发的同位素测量是否可以检测到饮食变化，方法是在不同的季节重新访问50名参与者，并收集第二套饮食记录和头发样本。初步数据收集显示，自给自足和非自给自足的Yupik食物之间存在强烈的同位素差异。研究参与者的招募接近完成，初步数据分析正在进行中。是的，爱斯基摩人尽管肥胖率相对较高，但与肥胖相关的慢性疾病水平较低。了解爱斯基摩人独特的饮食如何保护他们或使他们容易患病，需要实用的饮食模式生物标记物。这种饮食评估方法将专门为服务不足的尤皮克·爱斯基摩人社区使用，尽管该方法将适用于其他人群。 预计本项目研究对象的性别/少数群体构成不会发生变化。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. This research seeks to develop and validate a novel set of dietary biomarkers based on naturally-occurring isotopic differences among foods. Isotopic differences can distinguish foods from marine and terrestrial sources, foods grown at high and low latitude, and foods based on corn or sugar cane. Because Yup'ik Eskimos rely on a mix of subsistence and US market foods, they present an ideal population for testing these biomarkers of intake. First, we will test whether the isotopic signatures of key foods can be used to define nutritionally-relevant food groups, by collecting and analyzing multiple isotope signatures of commonly consumed foods. Secondly, we will enroll 100 individuals in an age and sex stratified design and compare intake measured by repeated diet records and by the isotope signatures of red blood cells, hair, and fingernails. Finally, we will test whether isotopic measurements of hair can detect dietary change, by revisiting 50 participants in a different season and collecting a second set of diet records and hair samples. Preliminary data collection reveals strong isotopic differences between subsistence and non subsistence Yup'ik foods. Enrollment of research participants is nearing completion, and preliminary data analyses are underway. Yup'ik Eskimos exhibit low levels of chronic, obesity-related disease, despite having relatively high rates of obesity. Understanding how the unique diet of Yup'ik Eskimos either protects or predisposes them to disease requires practical biomarkers of diet pattern. This approach to diet assessment will be developed specifically for use in underserved Yup'ik Eskimo communities, although the method will be adaptable to other populations. There are no expected changes in the gender/minority composition of research subjects for this project.