INBRE-2 MICROBIAL DETOXIFICATION OF POLYCHLORINATED BIPHENYLS (PCBS) IN SOIL

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 多氯联苯(PCbs)是一种持久性有机污染物，在食物链中生物积累，对人类健康构成威胁。在阿拉斯加，多氯联苯污染存在于许多以前使用过的防御工地(FUDS)，包括靠近自给自足人口的偏远地区。传统的印刷电路板清理方法极其昂贵，特别是在偏远地区。或者，生物修复是一种有吸引力的方法，可以解毒被多氯联苯污染的土壤，减少人类暴露。我们的主要目标是制定加速多氯联苯解毒的战略，利用阿拉斯加本土植物促进土壤中微生物多氯联苯降解的能力。以往的研究表明，某些植物可能会从根部释放次生芳香化合物，有效地促进根际环境中多氯联苯降解菌的长期生物刺激，这一过程被称为根修复。众所周知，阿拉斯加树种含有极高水平的芳香化合物，因此特别有希望促进多氯联苯污染场地的解毒，包括全州受污染的FUDS场地。利用微观世界研究和基于分子和培养的微生物学技术，再加上密集的化学分析和建模，我们将评估加速微生物多氯联苯脱毒和减少多氯联苯运输潜力的不同策略。将通过对多氯联苯进行化学分析，包括共面二恶英同系物和有毒对映体，然后根据已建立的单个同系物和对映体的毒性当量因数(TEF)计算多氯联苯的毒性当量商(TEQ)的变化，来评估毒性的减少。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Polychlorinated biphenyls (PCBs) are persistent organic pollutants that bioaccumulate in the food chain and pose risks to human health. In Alaska, PCB contamination is present in many formerly used defense sites (FUDS), including remote areas near subsistence-fed human populations. Conventional methods for PCB cleanup are extremely expensive, particularly in remote areas. Alternatively, bioremediation is an attractive means to detoxify PCB-contaminated soils and reduce human exposure. Our primary objective is to develop strategies for accelerating PCB detoxification that capitalize on the ability of native Alaskan plants to promote microbial PCB degradation in soil. Previous studies indicate that certain plants may release secondary aromatic compounds from their roots that effectively facilitate long-term biostimulation of PCB degrading bacteria in the rhizosphere, in a process known as rhizoremediation. Alaskan tree species are known to possess extremely high levels of aromatic compounds, and thus would be particularly promising for facilitating detoxification of PCB-contaminated sites, including contaminated FUDS sites across the state. Using microcosm studies and molecular and cultivation-based microbiological techniques, coupled with intensive chemical analyses and modeling, we will evaluate different strategies for accelerating microbial PCB detoxification and reducing potential for PCB transport. Reduction in toxicity will be evaluated using chemical analysis of PCBs, including coplanar dioxin-like congeners and toxic enantiomers, followed by calculations of the change in toxic equivalency quotient (TEQ) of PCBs based on the established toxic equivalency factor (TEF) of individual congeners and enantiomers.