Enabling Technologies to Macromolecular Crystallization

大分子结晶技术

• 批准号: 8260587
• 负责人: GEORGE T DETITTA
• 金额: $ 48.52万
• 依托单位: HAUPTMAN-WOODWARD MEDICAL RESEARCH INST
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-05-01 至 2015-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8260587
• 关键词: Area; Biological; Biology; Buffaloes; Chemicals; Communities; Complex; Crystallization; Development; Environment; Fostering; Goals; Graph; Growth; Individual; Informatics; Institutes; Instruction; Investigation; Laboratories; Life; Literature; Methods; Play; Production; Property; Protein Structure Initiative; Proteins; Research; Research Infrastructure; Research Personnel; Resources; Role; Science; Scientist; Services; Shapes; Structure; Synchrotrons; System; Technology; Testing; Time; Work; X ray diffraction analysis; X-Ray Crystallography; X-Ray Diffraction; base; beamline; improved; macromolecule; member; novel strategies; outreach; structural biology; structural genomics; success; tool

Structural biology, the study of the shapes of biologically important molecules, is a foundational science in the understanding of life; it is principally the result of single crystal diffraction studies. The project "Enabling Technologies for Macromolecular Crystallization" expands upon the work of the High Throughput Search Lab (HTSlab), a macromolecular crystallization facility at the Hauptman-Woodward Institute in Buffalo, NY. The HTSlab specializes in the development of high throughput methods to grow crystals of macromolecules suitable for diffraction studies; the formation of such crystals is a significant bottleneck in the science of structural biology. We will continue to partner with general biology, structural biology and structural genomics groups to undertake crystallization trials and enable analysis of these trials on as many as 1000 macromolecules each year. We will work in three areas: the provision of crystallization expertise and services for members of the three biology communities; the development and testing of crystallization methods and materials in a high throughput environment; and the integration of informatics services and tools for the analysis of crystallization results. We expect to work closely with the PSI community as it directs its further efforts toward a deeper understanding of the biology of complex systems.

结构生物学是对生物学上重要分子形状的研究，是一门基础科学 对生活的理解；这主要是单晶衍射研究的结果。该项目“启用 大分子结晶技术”在高吞吐量搜索实验室的工作上扩展 （HTSLAB），纽约州布法罗Hauptman-Woodward Institute的大分子结晶设施。这 HTSLAB专门从事高吞吐量方法的发展，以种植大分子的晶体 适合衍射研究；这种晶体的形成是科学的重要瓶颈 结构生物学。我们将继续与一般生物学，结构生物学和结构合作 基因组学小组进行结晶试验并启用对多达1000的试验的分析 每年大分子。我们将在三个领域工作：提供结晶专业知识和 为三个生物学社区成员的服务；结晶的开发和测试 高吞吐量环境中的方法和材料；以及信息学服务的整合 分析结晶结果的工具。我们希望与PSI社区紧密合作 将其进一步的努力引向更深入地了解复杂系统的生物学。