Diagonal Capillary Electrophoresis for Phosphorylation and Sialylation Analysis

用于磷酸化和唾液酸化分析的对角毛细管电泳

基本信息

  • 批准号:
    8306043
  • 负责人:
  • 金额:
    $ 28.5万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
  • 财政年份:
    2011
  • 资助国家:
    美国
  • 起止时间:
    2011-08-01 至 2015-07-31
  • 项目状态:
    已结题

项目摘要

Modified Abstract. This research group recently published the first description of diagonal capillary electrophoresis. Diagonal capillary electrophoresis is a form of two-dimensional capillary electrophoresis that employs identical separation modes in each dimension. The distal end of the first capillary incorporates an enzyme-based microreactor. Analytes that are not modified by the reactor will have identical migration times in the two capillaries and will generate spots that fall on the diagonal in a reconstructed two-dimensional electropherogram. Analytes that undergo enzymatic modification in the reactor will have a different migration time in the second capillary and will generate spots that fall off the diagonal in the electropherogram. Dephosphorylation and desialylation result in the loss of a negative charge, which is trivial to detect using capillary electrophoresis. In this proposal, we will develop diagonal capillary electrophoresis as a robust tool for the determination of the phosphorylation status of complex samples. The technology offers significant advantages compared with alternatives. Most importantly, the technology provides accurate estimates of the extent of modification without artifact due to differences in ionization efficiency of the native and modified peptides. Also, the technology is fully automated: once the sample is loaded into the instrument, no fraction collection, pipetting, or other sample manipulations are performed. Finally, the technology employs capillary electrophoresis, which appears to be better suited than reversed-phase liquid chromatography for the analysis of peptides containing charged modifications.
修改后的摘要。这个研究小组最近发表了对角线的第一个描述

项目成果

期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(5)

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Norman J Dovichi其他文献

Climbing the protein ladder
爬上蛋白质阶梯
  • DOI:
    10.1038/nbt1004-1242
  • 发表时间:
    2004-10-01
  • 期刊:
  • 影响因子:
    41.700
  • 作者:
    Norman J Dovichi
  • 通讯作者:
    Norman J Dovichi

Norman J Dovichi的其他文献

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{{ truncateString('Norman J Dovichi', 18)}}的其他基金

Advances in bioanalysis
生物分析的进展
  • 批准号:
    10605342
  • 财政年份:
    2020
  • 资助金额:
    $ 28.5万
  • 项目类别:
Advances in bioanalysis
生物分析的进展
  • 批准号:
    10396021
  • 财政年份:
    2020
  • 资助金额:
    $ 28.5万
  • 项目类别:
Quantitative proteomics of Xenopus oocytes, eggs, blastomeres, and nuclear transplants
非洲爪蟾卵母细胞、卵子、卵裂球和核移植物的定量蛋白质组学
  • 批准号:
    8937359
  • 财政年份:
    2015
  • 资助金额:
    $ 28.5万
  • 项目类别:
Quantitative proteomics of Xenopus oocytes, eggs, blastomeres, and nuclear transplants
非洲爪蟾卵母细胞、卵子、卵裂球和核移植物的定量蛋白质组学
  • 批准号:
    9116239
  • 财政年份:
    2015
  • 资助金额:
    $ 28.5万
  • 项目类别:
Two-dimensional Capillary Electrophoresis - SELEX
二维毛细管电泳 - SELEX
  • 批准号:
    8453389
  • 财政年份:
    2011
  • 资助金额:
    $ 28.5万
  • 项目类别:
Diagonal Capillary Electrophoresis for Phosphorylation and Sialylation Analysis
用于磷酸化和唾液酸化分析的对角毛细管电泳
  • 批准号:
    8710257
  • 财政年份:
    2011
  • 资助金额:
    $ 28.5万
  • 项目类别:
Technology for single cell proteomics and its application to the analysis of single Xenopus blastomeres
单细胞蛋白质组学技术及其在非洲爪蟾单个卵裂球分析中的应用
  • 批准号:
    9198018
  • 财政年份:
    2011
  • 资助金额:
    $ 28.5万
  • 项目类别:
Two-dimensional Capillary Electrophoresis - SELEX
二维毛细管电泳 - SELEX
  • 批准号:
    8164257
  • 财政年份:
    2011
  • 资助金额:
    $ 28.5万
  • 项目类别:
Two-dimensional Capillary Electrophoresis - SELEX
二维毛细管电泳 - SELEX
  • 批准号:
    8286819
  • 财政年份:
    2011
  • 资助金额:
    $ 28.5万
  • 项目类别:
Diagonal Capillary Electrophoresis for Phosphorylation and Sialylation Analysis
用于磷酸化和唾液酸化分析的对角毛细管电泳
  • 批准号:
    8534195
  • 财政年份:
    2011
  • 资助金额:
    $ 28.5万
  • 项目类别:

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