Cytogenetics

9.3.1摘要：细胞遗传学 导演：Betsy Hirsch博士。 细胞遗传学共享资源提供细胞培养、核型分析和分子细胞遗传学 服务(包括荧光原位杂交和基于寡核苷酸阵列的比较生理学 杂交)，用于人类和动物细胞的评估。细胞遗传学核心已经研究了20种不同的 代表癌症中心内五个不同项目的调查人员。在过去的五年里， CORE扩大了其服务范围，以满足这些调查人员的需求。例子包括详细的 小鼠和人细胞染色体断裂研究对DNA修复相关基因的评估 和保持染色体稳定性，序列G显带到SKY和FISH用于作图 转座子插入位点和阵列-CGH用于评估基因组拷贝数的细微变化。 这些分析为研究人员提供了新的信息，这对他们未来的研究至关重要 程序。此外，核心还提供了数百种传统的核型分析，作为关键 涉及基因操纵的细胞或细胞群体的实验的质量控制措施 已经在体外扩增了。核型验证通常是发表这类工作的先决条件： 作为一个不断扩大的技术剧目，核心还侧重于在实验中提供咨询援助 设计和选择细胞遗传学和/或分子细胞遗传学技术。大量的时间被投入到 与研究人员举行个人会议，讨论分析结果和解释，并提供 未来研究的方向。

9.3.1 ABSTRACT: CYTOGENETICS Director: Betsy Hirsch, Ph.D. The Cytogenetics shared resource provides cell culture, karyotype analysis, and molecular cytogenetic services (including fluorescence-in-situ-hybridization and oligonudeotide array based comparative gendmic hybridization) for evaluation of human and animal cells. The Cytogenetics Core has worked with 20 different investigators representing five different programs within the Cancer Center. Over the past five years, the Core has expanded its services to meet the needs of these investigators. Examples include detailed chromosome breakage studies of mouse and human cells for evaluation of genes involved in DMA repair and maintenance of chromosome stability, sequential G-banding to SKY and FISH for mapping of transposon insertion sites, and array-CGH for the evaluation of subtle genomic copy number changes. These analyses have provided novel information to investigators that is pivotal to their future research programs. In addition, the Core has provided hundreds of conventional karyotype analyses as critical quality control measures for experiments involving genetically manipulated cells or populations of cells that have been expanded in-vitro. Karyotype verification is often a prerequisite for publication of such work: With an expanding repertoire of techniques, the Core has also focused on consultative assistance in experimental design and selection of cytogenetic and/or molecular cytogenetic techniques. Significant time is devoted to personal meetings with researchers to discuss the results and interpretation of analyses, and provide direction for future research.