Electrophysiological Assessment

电生理评估

• 批准号: 8382159
• 负责人: Carlos T Cepeda
• 金额: $ 10.55万
• 依托单位: UNIVERSITY OF CALIFORNIA LOS ANGELES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/8382159
• 关键词: Animal Experiments; Animal Model; Animals; Brain; Cellular biology; Collaborations; Consultations; Cultured Cells; D Cells; Data; Development; Developmental Disabilities; Electrophysiology (science); Faculty; Image; Individual; Instruction; Intellectual functioning disability; Laboratories; Mental Retardation and Developmental Disabilities Research Centers; Modeling; Monitor; Mus; Mutation; Neurons; Persons; Physiological; Quality Control; Rattus; Research; Research Personnel; Role; Schedule; Services; Slice; Time; Training; base; cellular imaging; experience; meetings; research study; tissue fixing

The Core will be administered by Dr. Michael Levine. His role will be to oversee all Core services. Dr. Cepeda will provide training and instruction in electrophysiological assessment and in some cases will help perform the physiological experiments. Core services will be accessed first by consultation with Drs. Levine and Cepeda. The purpose of the consultation will be to determine which services are needed and when and how they might best be provided. Drs. Levine and Cepeda will meet with the investigator to determine the services needed and set up a time-line for provicling the service. Thus, these services are available on a firstcome, first-served basis. IDDRC investigators that need aid in performing electrophysiological analyses will have access to these Core services which are not to our knowledge presently available at UCLA except when collaborations are set up between investigators. Training and/or use of the Core set-ups will be performed according to an individualized schedule that depends on the sophistication and previous experience of the Core user. Electrophysiological experiments are labor intensive and time-consuming. It is our experience that only one investigator (or perhaps two at most depending on the services they need) will be able to use the Core at one time. We expect that most projects will require one to two months to complete (depending on availability of cultured cells and animals when slices are made), although training in electrophysiology may take longer depending on the ability of the person being trained. Once an individual is trained, they will be able to use the facility to perform experiments. The experiments and the data collected will be monitored both by Drs. Levine and Cepeda. Our experience is that weekly meetings with the investigator collecting the data are sufficient for quality control and assessment. The Physiological Assessment Core will interact directly with Core D, Cell Biology and Cellular Imaging to coordinate electrophysiological and imaging experiments on the same animals or on fixed tissue after electrophysiology is performed and Core E, Animal Models Core to determine availability of animals for experiments.

核心将由迈克尔·莱文博士管理。他的角色将是监督所有核心服务。Dr。 CEPEDA将提供电生理评估方面的培训和指导，在某些情况下还将帮助进行生理实验。核心服务将首先通过与Levine博士和Cepeda博士协商获得。协商的目的将是确定需要哪些服务，以及何时和如何最好地提供这些服务。Levine博士和Cepeda博士将与调查人员会面，以确定所需的服务，并设定提供服务的时间表。因此，这些服务以先到先得的方式提供。 需要帮助进行电生理分析的IDDRC调查人员将可以使用这些核心服务，据我们所知，加州大学洛杉矶分校目前没有这些服务，除非调查人员之间建立了合作。核心设置的培训和/或使用将根据核心用户的复杂程度和以前的经验，按照个性化的时间表进行。 电生理实验是一项费时费力的实验。根据我们的经验，一次只有一名调查员(或最多两名调查员，取决于他们所需的服务)能够使用核心。我们预计，大多数项目将需要一到两个月的时间来完成(取决于切片制作时可获得的培养细胞和动物)，尽管电生理学培训可能需要更长的时间，这取决于受训者的能力。一旦一个人接受了培训，他们将能够使用该设施进行实验。实验和收集的数据将由Levine博士和Cepeda博士共同监督。我们的经验是，每周与收集数据的调查员举行会议就足以进行质量控制和评估。 生理学评估核心将直接与核心D，细胞生物学和细胞成像，以协调电生理后相同的动物或固定组织上的电生理和成像实验，以及核心E，动物模型核心，以确定动物可用于实验。