BIOCHEMICAL AND MOLECULAR BIOLOGY CORE LABORATORY

生化与分子生物学核心实验室

• 批准号: 8305024
• 负责人: Zeljko J. Bosnjak
• 金额: $ 38.1万
• 依托单位: MEDICAL COLLEGE OF WISCONSIN
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-08-01 至 2013-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8305024
• 关键词: Anesthetics; Animal Model; Biochemical; Biological Assay; Cardiac; Cardiovascular system; Chemicals; Consultations; Core Facility; Equipment; Functional disorder; Gene Expression; Guidelines; Human Resources; Laboratories; Measures; Messenger RNA; Metabolic; Modeling; Molecular; Molecular Biology; Molecular and Cellular Biology; Myocardial Infarction; Procedures; Program Research Project Grants; Quality Control; Records; Research Personnel; Running; Sampling; Services; Small Interfering RNA; Techniques; Technology; Time; Training; computerized; experience; meetings; preconditioning; programs; training project

This Biochemical and Molecular Core facility, under the direction of Dr. David Harder with the assistance of Jayashree Narayanan, will provide a centralized professional service to all three projects involved in this Program. The staff will be trained by the project leaders or specialized personnel to perform a variety of procedures required in this Program Project Grant. This facility will maintain complete, computerized and centralized records and well-defined quality control guidelines for all assays. In addition new assays will be developed by Dr. Harder in consultation with investigators to meet the changing needs and directions of the Program. New assays that have thus far been developed by the Core include siRNA technology to silence gene expression, real time PCR to quantitate mRNA levels, metabolic models to record endothelial dysfunction and animal models to measure myocardial infarction. Dr. Harder and Ms. Narayanan have first hand experience in running core laboratories for two other Program Projects. The Core facility is located in the Cardiovascular Center which is in close proximity to the laboratories of the investigators for all three projects.

这个生化和分子核心设施，在大卫哈德博士的指导下， 在Jayashree Narayanan的协助下，将为所有三个项目提供集中的专业服务 参与这个计划。工作人员将由项目负责人或专业人员进行培训， 本计划项目补助金所需的各种程序。该设施将保持完整， 计算机化和集中的记录以及所有测定的明确质量控制指南。此外 哈德博士将与研究人员协商，开发新的检测方法，以满足不断变化的需求 和方案的方向。 到目前为止，Core开发的新检测方法包括siRNA技术， 基因表达，真实的时间PCR定量mRNA水平，代谢模型记录内皮细胞 功能障碍和动物模型来测量心肌梗死。哈德博士和纳拉亚南女士首先 有为其他两个项目运行核心实验室的经验。核心设施位于 心血管中心，靠近所有三个研究者的实验室 项目