Laboratory

脑血管畸形的综合研究 实验室核心（核心C）将为该计划提供基础设施和实验方法。 核心C将作为一个小动物手术实验室，细胞和分子生物学实验室，成像实验室， 分析实验室和转基因动物育种单位。PPG内体内实验的关键部分 将在核心中进行，从而为实验提供质量控制和一致性。 我们的具体目标是：（目标1）为实验室项目提供多种动物模型，包括 可重复的血管发育不良模型，流动诱导的适应性外向动脉重塑模型，和 成熟啮齿动物脑中的人-鼠移植模型（项目2、3和4）;（目标2）继续 改进我们现有的模型，并利用生长因子的操纵来开发新的动物模型， 刺激、遗传背景和血流动力学操纵;（目标3）促进人类 标本，并作为人类手术标本的临床组织库。 核心C将协助设计实验，撰写实验提案，并解决方法论问题。 它还将为讨论和解释结果提供一个论坛。

Integrative Study of Brain Vascular Malformations The Laboratory Core (Core C) will provide infrastructure and experimental methodology for the program. Core C will function as a small animal surgical lab, a cellular and molecular biology lab, an imaging analysis lab, and a transgenic animal breeding unit. Key portions of the in vivo experiments within the PPG will be performed in the Core, thus providing quality control and consistency for the experiments. Our Specific Aims are: (Aim 1) Provide several animal models to the laboratory projects, including reproducible vascular dysplasia models, a flow-induced adaptive outward arterial remodeling model, and human-to-mouse transplantation model in the mature rodent brain (Projects 2, 3 and 4); (Aim 2) Continue to refine our current models and develop novel animal models using manipulation of growth factor stimulation, genetic background and hemodynamic manipulation; (Aim 3) Facilitate transfer of human specimens and serve as the clinical tissue bank for human surgical specimens. Core C will assist in designing the experiments, writing experimental proposals, and solving methodological problems; it will also provide a forum for discussing and interpreting results.