High-throughput gene disruption in zebrafish using retroviral integration

摘要 斑马鱼已被广泛用作研究脊椎动物发育和遗传学的模式动物。公 建立了斑马鱼基因的遗传研究可以提供对保守的功能的见解， 脊椎动物的基因，从而导致更好地了解人类疾病和健康。和斑马鱼在一起 基因组测序计划即将完成，非常希望所有的基因预测由 测序还将携带易于用于基因功能研究的突变。为了实现这一目标，我们 已经开发了一种高效和精简的平台，使用前病毒插入加上高通量 测序和作图技术，以诱变基本上所有可识别的候选基因， 斑马鱼基因组在这个项目中，我们建议通过以下方式为公共研究社区提供遗传工具： 构建斑马鱼品系文库，其中约12，000个斑马鱼基因已被破坏 插入了逆转录病毒所有产生的资源将提供给社区， 出版前的成本。

Abstract Zebrafish has been widely used as a model animal to study vertebrate development and genetics. It is well established that genetic studies of zebrafish genes can provide insights into the function of the conserved vertebrate genes and therefore lead to better understanding of human diseases and health. With the zebrafish genome sequencing project nearly completed, it is highly desirable that all of the genes predicated by sequencing will also carry an mutation that readily available for gene function studies. Towards this goal, we have developed an efficient and streamlined platform using proviral insertions coupled with high-throughput sequencing and mapping technologies to mutagenize essentially all of the identifiable candidate genes in zebrafish genome. In this project we propose to provide genetic tools to the public research community by constructing a library of zebrafish strains in which approximately 12,000 zebrafish genes have been disrupted with a retrovirus insertion. All the resources generated will be made available to the community with a minimal cost before publication.