DIACEST Islet Cell Capsules for Immunoprotection, MR Detection, and pH Sensing
用于免疫保护、MR 检测和 pH 传感的 DIACEST 胰岛细胞胶囊
基本信息
- 批准号:8323997
- 负责人:
- 金额:$ 34.82万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2010
- 资助国家:美国
- 起止时间:2010-09-22 至 2014-08-31
- 项目状态:已结题
- 来源:
- 关键词:Acute Liver FailureAlginatesAmino AcidsAntibodiesApoptosisArginineAttentionBiocompatibleCell LineCell SurvivalCell TherapyCell physiologyCellsChemicalsClinicClinicalColorContrast MediaCrosslinkerDetectionDiabetic mouseDiscriminationDiseaseEncapsulatedEnvironmentFirefly LuciferasesGoalsHemophilia AHumanImageImageryIn VitroIndividualInsulinInsulin-Dependent Diabetes MellitusIslet CellIslets of LangerhansIslets of Langerhans TransplantationLabelLibrariesLifeLipidsLiposomesMagnetic ResonanceMagnetic Resonance ImagingMalignant NeoplasmsMeasuresMembraneMesenchymalMesenchymal Stem CellsMetalsMethodsMicroencapsulationsMonitorMusParkinson DiseasePatientsPeptidesPhysiologicalPopulationProductionReportingSignal TransductionSourceStagingStem cellsStreptozocinTechniquesTestingTherapeutic immunosuppressionTimeToxic effectTreatment ProtocolsValidationbasecapsulecell capsuledesignfunctional statusglycemic controlin vivoinsulin secretioninsulinomaisletmouse modelpre-clinicalpreclinical studypreventpublic health relevanceremote sensingsmall molecule librariessuccess
项目摘要
DESCRIPTION (provided by applicant): Good glycemic control is critical in reducing the end-stage complications of Type I Diabetes Mellitus (TIDM) and, for this reason, islet transplantation (ITx) has received considerable attention in recent years, with one of the complications in the treatment involves finding an effective means to prevent rejection. Immuno-isolation of islets provides a means to reduce or avoid immunosuppressive therapy altogether. In the most commonly used method of immuno-isolation, microencapsulation, individual islets are surrounded with thin alginate membranes that are permeable to insulin and metabolites, but are impermeable to native antibodies. However, the viability and in particular the insulin secretion of these islets are critical for the success of the therapy, and would be important to monitor for refinement of the treatment protocol. This proposal is focused on the production of micron sized CEST MR detectable alginate capsules containing both islet and mesenchymal stem cells which are appropriate for the treatment of T1DM. These capsules will have CEST MR contrast agents built into the capsule to allow: 1) detection, 2) discrimination between encapsulated islet and mesynchymal stem cells and 3) remote sensing of the pH of the environment. The source of the CEST contrast will be 6-24 amino acid long peptides and CEST liposomes embedded within the alginate. We will perform pre-clinical studies using a nude based mouse model of diabetes to allow monitoring of the status of encapsulated islets/ mesenchymal cells through firefly luciferase expression.
PUBLIC HEALTH RELEVANCE: Islet transplantation (ITx) has received considerable attention in recent years for treatment of Type 1 Diabetes Melitus (T1DM). This proposal is focused on the production of immunoprotective islet cell capsules which are appropriate for treatment and allow MR monitoring of the pH these islets using biodegradable CEST contrast agents.
描述(由申请人提供):良好的血糖控制对于减少I型糖尿病(TIDM)的终末期并发症至关重要,因此,近年来胰岛移植(ITx)受到了相当大的关注,治疗中的并发症之一涉及寻找有效的方法来预防排斥反应。胰岛的免疫隔离提供了一种减少或完全避免免疫抑制治疗的方法。在最常用的免疫隔离方法微囊化中,单个胰岛被薄藻酸盐膜包围,该膜可渗透胰岛素和代谢物,但不可渗透天然抗体。然而,这些胰岛的活力,特别是胰岛素分泌对于治疗的成功是至关重要的,并且对于监测治疗方案的改进是重要的。该提案的重点是生产微米尺寸的CEST MR可检测的藻酸盐胶囊,其含有适于治疗T1 DM的胰岛和间充质干细胞。这些胶囊将具有内置于胶囊中的CEST MR造影剂,以允许:1)检测,2)在包封的胰岛和间充质干细胞之间进行区分,以及3)远程感测环境的pH。CEST造影剂的来源将是6-24个氨基酸长的肽和嵌入藻酸盐内的CEST脂质体。我们将使用糖尿病的裸小鼠模型进行临床前研究,以允许通过萤火虫荧光素酶表达监测包封的胰岛/间充质细胞的状态。
公共卫生相关性:近年来,胰岛移植(ITx)在治疗1型糖尿病(T1 DM)方面受到了相当大的关注。该提案的重点是生产免疫保护性胰岛细胞胶囊,其适用于治疗,并允许使用可生物降解的CEST造影剂对这些胰岛的pH值进行MR监测。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
数据更新时间:{{ journalArticles.updateTime }}
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
数据更新时间:{{ journalArticles.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ monograph.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ sciAawards.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ conferencePapers.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ patent.updateTime }}
MICHAEL T MCMAHON其他文献
MICHAEL T MCMAHON的其他文献
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
{{ truncateString('MICHAEL T MCMAHON', 18)}}的其他基金
Developing MRI contrast agents to detect progression in renal disease as a consequence of acidema
开发 MRI 造影剂来检测酸血症导致的肾脏疾病的进展
- 批准号:
10551303 - 财政年份:2020
- 资助金额:
$ 34.82万 - 项目类别:
Developing MRI contrast agents to detect progression in renal disease as a consequence of acidema
开发 MRI 造影剂来检测酸血症导致的肾脏疾病的进展
- 批准号:
10337274 - 财政年份:2020
- 资助金额:
$ 34.82万 - 项目类别:
Multi-Color Exchange Transfer Imaging of Drug Delivery Nanocarriers
药物输送纳米载体的多色交换转移成像
- 批准号:
8479358 - 财政年份:2011
- 资助金额:
$ 34.82万 - 项目类别:
Multi-Color Exchange Transfer Imaging of Drug Delivery Nanocarriers
药物输送纳米载体的多色交换转移成像
- 批准号:
8292578 - 财政年份:2011
- 资助金额:
$ 34.82万 - 项目类别:
Multi-Color Exchange Transfer Imaging of Drug Delivery Nanocarriers
药物输送纳米载体的多色交换转移成像
- 批准号:
8677891 - 财政年份:2011
- 资助金额:
$ 34.82万 - 项目类别:
Multi-Color Exchange Transfer Imaging of Drug Delivery Nanocarriers
药物输送纳米载体的多色交换转移成像
- 批准号:
8305994 - 财政年份:2011
- 资助金额:
$ 34.82万 - 项目类别:
DIACEST Islet Cell Capsules for Immunoprotection, MR Detection, and pH Sensing
用于免疫保护、MR 检测和 pH 传感的 DIACEST 胰岛细胞胶囊
- 批准号:
8023872 - 财政年份:2010
- 资助金额:
$ 34.82万 - 项目类别:
DIACEST Islet Cell Capsules for Immunoprotection, MR Detection, and pH Sensing
用于免疫保护、MR 检测和 pH 传感的 DIACEST 胰岛细胞胶囊
- 批准号:
8525116 - 财政年份:2010
- 资助金额:
$ 34.82万 - 项目类别:
DIACEST Islet Cell Capsules for Immunoprotection, MR Detection, and pH Sensing
用于免疫保护、MR 检测和 pH 传感的 DIACEST 胰岛细胞胶囊
- 批准号:
8146032 - 财政年份:2010
- 资助金额:
$ 34.82万 - 项目类别:
相似海外基金
Étude des interactions de sorption et de séquestration de polluants sur des alginates
海藻酸盐污染物吸附与封存相互作用研究
- 批准号:
571945-2022 - 财政年份:2022
- 资助金额:
$ 34.82万 - 项目类别:
University Undergraduate Student Research Awards
Engineering an Islet Thread from zwitterionically modified alginates for type 1 diabetes
利用两性离子改性藻酸盐设计胰岛丝,用于治疗 1 型糖尿病
- 批准号:
9910390 - 财政年份:2018
- 资助金额:
$ 34.82万 - 项目类别:
Engineering an Islet Thread from zwitterionically modified alginates for type 1 diabetes
利用两性离子改性藻酸盐设计胰岛丝,用于治疗 1 型糖尿病
- 批准号:
10402773 - 财政年份:2018
- 资助金额:
$ 34.82万 - 项目类别:
ALGIPRO - Alginates by Production Scale Fermentation and Epimerisation
ALGIPRO - 通过生产规模发酵和差向异构化生产海藻酸盐
- 批准号:
102148 - 财政年份:2016
- 资助金额:
$ 34.82万 - 项目类别:
Collaborative R&D
Bioactive Alginates and Obesity
生物活性藻酸盐与肥胖
- 批准号:
BB/G00563X/1 - 财政年份:2008
- 资助金额:
$ 34.82万 - 项目类别:
Research Grant