Optimization of Core Animal Services at The Ohio State University

描述（由申请人提供）：俄亥俄州立大学（OSU）申请50万美元，用于在主校区翻新现有的动物园内，为转基因小鼠（GEM）提供检疫设施，并为转基因斑马鱼提供住房，以支持该机构的整个生物医学研究计划。在上一个财政年度，俄勒冈州立大学的老鼠和斑马鱼用户已经获得了超过6900万美元的研究经费。目前超过75%的小鼠和斑马鱼研究人员使用基因工程模型。Rightmire Hall （RH）动物设施建于1990-91年，位于俄勒冈州立大学西校区，位于俄勒冈州立大学主校区以西一英里处。RH位于地下室，有2458平方英尺的空间，包括四个动物饲养室，机架洗衣机，饲料，床上用品和干净的笼子存储，以及一个大的小程序空间。该提案将升级动物空间，提供一次性笼子系统，以提高GEM检疫的生物安全性，并为俄勒冈州立大学的神经科学研究提供必要的斑马鱼设施。俄勒冈州立大学致力于支持NIH的期望，即在NIH资助的帮助下产生的小鼠资源将及时分配并与科学界共享。进口到俄勒冈州立大学的大多数小鼠都是独特的GEM，无法从商业来源获得。从非商业来源进口的GEM必须进行检疫，以避免室内菌落受到污染。新技术的使用提高了生产转基因斑马鱼品系的效率；然而，这些改进的方法也需要大量的空间才能有效。目前的斑马鱼设施是由多个研究人员在一个研究者维护的空间内共享的，没有扩展能力来产生正在进行的研究所需的新的转基因和突变系。本次提案要求的设备包括9个带一次性笼子的innoi - vive单面通风鼠架和一个5架斑马鱼水生栖息地系统。一次性笼子将增加动物住房的可用空间；减少人员时间，并提供符合俄勒冈州立大学环境责任承诺的绿色替代方案。所要求的224缸5架水产架将容纳额外的10 640条鱼。还将建立两个独立的程序室，为斑马鱼的基因操作和隔离中的啮齿动物的群体维持提供空间。通过进行这些翻新和设备采购，我们期望能够实现我们的目标，即重新利用和现代化一个小型的老动物饲养场，提供一个集中有效的啮齿动物检疫设施，并增加管理集中的斑马鱼居住空间。

DESCRIPTION (provided by applicant): The Ohio State University (OSU) requests $0.5M to renovate an existing vivarium to generate quarantine facilities on main campus for genetically engineered mice (GEM) and generate housing for transgenic zebrafish in support of the institution's entire biomedical research program. During the last fiscal year, mouse and zebrafish users at OSU have generated grants supporting over $69M in research funding. Over 75% of our current mouse and zebrafish researchers use genetically engineered models. The Rightmire Hall (RH) animal facility was built in 1990-91 and is located on the OSU West Campus one mile west of the OSU main campus. RH is located in the basement with 2,458 ft2 of space containing four animal holding rooms, rack washer, feed, bedding and clean cage storage, and a large minor procedure space. This proposal will upgrade the vivarium space, provide disposable caging systems to enhance biosecurity at the GEM quarantine and generate necessary zebrafish facilities in support of Neuroscience focused research at OSU. OSU is committed to upholding the NIH expectation that mouse resources generated with the aid of NIH funding will be distributed and shared with the scientific community in a timely manner. The majority of the mice imported into OSU are unique GEM and are not available from commercial sources. Quarantine is required for the import of GEM from non-commercial sources to avoid contamination of in house colonies. The use of new technologies has increased the efficiency of generating transgenic zebrafish lines; however, these improved approaches also require a substantial amount of space to be effective. The current zebrafish facility is shared by multiple researchers within an investigator maintained space with no expansion ability to generate the new transgenic and mutant lines required for ongoing research. The equipment requested in this proposal includes nine Inno-Vive single sided ventilated mouse racks with disposable cages and one 5-rack Aquatic Habitats system for zebrafish. Disposable caging will increase available space for animal housing; decrease personnel time and provide a green alternative consistent with OSU commitment to environmental responsibility. The requested 224 tank 5-shelf aquatic rack will accommodate an additional 10,640 fish. Two separate procedure rooms will also be generated to provide space for the genetic manipulation of zebrafish and for the colony maintenance of rodents in quarantine. By undertaking these renovations and equipment purchases, we anticipate being able to meet our goal of creating repurposing and modernizing a small aged vivarium, providing a centralized efficient rodent quarantine facility, and adding zebrafish housing space that is administratively centralized.