CORE SERVICE FOR FLOW CYTOMETRY

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 流式细胞术核心实验室仪器包括2台Becton-Dickinson（BD）FACSCalibur、1台BD LSRII和1台BD FACSAria。FACSCalibur能够进行6个参数分析（4种颜色加上前向和侧向散射）。它们用于四色采集、使用羧基荧光素二乙酸琥珀酰亚胺酯（CFSE）的细胞增殖研究和细胞倍性测定。LSRII和FACSAria均用于多参数样品测定。每个都是数字平台，包含3个激光器，能够进行14个参数分析（12种颜色加上前向和侧向散射）。FACSAria也是一种高速细胞分选仪，能够同时以极高的纯度无菌分选多达4个亚群。每个调查人员的需求各不相同。一些研究者利用了核心实验室提供的全面服务：从单克隆抗体选择到数据采集。大多数研究者处理自己的样本，为流式细胞术采集做准备。核心实验室提供有限的数据分析;大多数研究者进行自己的数据分析。原始数据文件通过安全的杜兰国家灵长类动物研究中心服务器分发给各个研究者。流式细胞术实验室提供4种分析服务。2010年的服务总数如下：Calibur = 4977;多色= 17106;排序= 47和TruCount = 1648。向核心调查员和附属调查员提供了这些服务。艾滋病研究计划是核心的最大用户。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The Flow Cytometry Core Laboratory instrumentation includes 2 Becton-Dickinson (BD) FACSCaliburs, a BD LSRII and a BD FACSAria. The FACSCaliburs are capable of 6 parameter analysis (4 colors plus forward and side scatter). They are used for 4-color acquisitions, cell proliferation studies using carboxy-fluorescein diacetate succinimidyl ester (CFSE) and cell ploidy determinations. Both the LSRII and the FACSAria are used for multiparameter sample determinations. Each is digital platform containing 3 lasers and capable of 14 parameter analysis (12 colors plus forward and side scatter). The FACSAria is also a high speed cell sorter, capable of aseptically sorting up to 4 subpopulations simultaneously with exceptional purity. The needs of individual investigators varied. Some investigators utilized the comprehensive services offered by the core lab: from monoclonal antibody selection through data acquisition. The majority of investigators processed their own samples in preparation for flow cytometry acquisition. The core lab provides limited data analysis; most investigators perform their own data analysis. Raw data files are distributed to the various investigators via a secure Tulane National Primate Research Center server. Flow Cytometry Laboratory provided services with 4 types of analysis. Service total for 2010 are as follows: Calibur = 4977; Multicolor = 17106; Sort = 47 and TruCount = 1648. These services were provided to both core and affiliate investigators. The AIDS research program is the largest user of the Core.