Clinical Specimen, Data Collection, and Expression Array Core

临床样本、数据收集和表达阵列核心

• 批准号: 8232987
• 负责人: Ingrid Adele Holm
• 金额: $ 17.74万
• 依托单位: BOSTON CHILDREN'S HOSPITAL
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-09-25 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/8232987
• 关键词: Bioinformatics; Biological Markers; Biopsy Specimen; Blood specimen; Cataloging; Catalogs; Cell Therapy; Cells; Centronuclear myopathy; Clinical; Clinical Services; Collaborations; Complementary DNA; DYSF gene; Data; Data Analyses; Data Collection; Databases; Diagnostic; Disease; Embryo; Enrollment; Fishes; Functional disorder; Gene Expression; Genes; Goals; Human; Label; Laboratories; Messenger RNA; Modeling; Molecular Profiling; Monitor; Muscle; Muscle Cells; Muscular Dystrophies; Mutation; Myopathy; Neuromuscular Diseases; Participant; Pathogenesis; Patients; Phenotype; Population; Preparation; Principal Investigator; Proteins; Quality Control; Reagent; Recording of previous events; Sampling; Specimen; Standardization; Techniques; Technology; Tissue Banks; Tissue Sample; Tissues; Transgenes; Zebrafish; design; disease-causing mutation; feeding; gene discovery; gene therapy; human fetus tissue; insight; meetings; myotubularin; novel strategies; programs; sample collection; tool; web site

The four projects in this Program Project share the common theme of developing newer approaches to cell and gene therapy, and using zebrafish models to accelerate improvements in understanding and treating the muscular dystrophies. The Program Pi'shave a long and extensive history of interaction and have exchanged many ideas, biopsy samples, and other reagents over many years. This Program Project further strengthens these collaborations to produce synergistic results that are beyond the scope of any one laboratory. The services of the Clinical Specimen, Data Collection, and Expression Array Core (Core B) are an important means by which the Program Project will meet these goals. Core B allows for increased efficiency and provides standardization to the analysis of data for each Project and the Program overall. Toward this end, the Aims of Core B are designed to maximize efficiency and minimize both administrative and technical effort and expense. Aim 1. To carry out all activities necessary for participant recruitment into the Program Projects, including the following: a) identify and enroll participants for the Program Project; b) acquire comprehensive data and specimens from participants, including clinical information, blood samples, and muscle tissue samples; c) acquire control tissues and specimens; d) catalogue and track the clinical and diagnostic data in a database; and e) maintain the HNDP web site (Projects served: 1, 2, 3, 4, Core C). Aim 2. To perform gene expression array analysis for the Program Project: a) Prepare zebrafish embryos and whole fish, cells, and muscle samples for gene expression analysis, isolate mRNA, and synthesize labeled cDNA/cRNA for hybridization to the Affymetrix GeneChip array and/or the Illumina Sentrix BeadChip; b) Hybridize cDNA to the Affymetrix GeneChip array and/or the Illumina Sentrix BeadChip; and c) Transfer gene expression data to ProjectManager, a HNDP database, for analysis in Core C. (Projects served: 1, 2, 3, Core C). Core B will interact extensively with Core C, where much of the analysis of the gene expression data will take place.

这个项目中的四个项目都有一个共同的主题，那就是开发新的细胞方法