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Mouse models of Desmoplastic Small Round Cell Tumor and Ewings sarcoma

促结缔组织增生性小圆细胞瘤和尤文氏肉瘤的小鼠模型

基本信息

批准号：
8553548
负责人：
Sean Lee
金额：
$ 38.62万
依托单位：
NATIONAL INSTITUTE OF DIABETES AND DIGESTIVE AND KIDNEY DISEASES
依托单位国家：
美国
项目类别：
财政年份：
资助国家：
美国
起止时间：
至
项目状态：
未结题

项目摘要

Desmoplastic small round cell tumor (DSRCT) is a rare but extremely aggressive cancer arising in young adolescents in the abdominal area. In all cases of DSRCT examined to date, a chromosomal translocation involving the Ewings Sarcoma gene (EWS) and the Wilms tumor suppressor gene, WT1, results in the fusion of the two genes, generating a novel transcription factor. In Ewing's sarcoma, which is a different type of pediatric tumor, EWS gene is fused to the Fli-1 gene, an ETS-family of transcription factor. Our interest is focused on understanding the molecular functions of EWS/WT1 and EWS/Fli1 gene products and how they contribute to tumorigenesis in their respective cancers. Towards this goal, we are currently developing mouse models of DSRCT and Ewing's sarcoma to better understand the etiology and the development of these tumors in the context of living organism. Aim 1. We will generate mouse models of these cancers with similar chromosomal tranlocations that are found in DSRCT and Ewing's sarcoma by using gene targeting approach. Successfully targeted mouse ES cells will then be injected to generate mice harboring these chromosomal translocations. Aim 2. We will examine the mice for the formation of spontaneous tumors and also to introduce secondary mutations by intercross with other cancer-prone animal models in hopes of accelerating tumorigenesis. Aim3. We will generate cell lines expressing the EWS/WT1 and EWS/Fli-1 translocations, either from the animals or from the tumors that are formed, and perform expression profiling analysis as well as proteomics (mass spectrometry) to identify tumorigenic pathways and interacting proteins that drive transformation. Our mouse models will help elucidate molecular mechanisms of these pediatric cancers and the knowledge gained will also shed light on the other adult tumors with similar characteristics. Our models will also be invaluable for the development of new therapeutics against DSRCT and Ewing'sarcoma.

促结缔组织增生性小圆细胞肿瘤（DSRCT）是一种罕见的，但非常积极的癌症发生在年轻的青少年在腹部地区。在迄今为止检查的所有DSRCT病例中，涉及尤文氏肉瘤基因（EWS）和Wilms肿瘤抑制基因WT 1的染色体易位导致两个基因融合，产生一种新的转录因子。在尤文氏肉瘤中，这是一种不同类型的儿科肿瘤，EWS基因融合到Fli-1基因，一个ETS家族的转录因子。我们的兴趣集中在了解EWS/WT1和EWS/Fli1基因产物的分子功能，以及它们如何在各自的癌症中促进肿瘤发生。为了实现这一目标，我们目前正在开发DSRCT和尤文肉瘤的小鼠模型，以更好地了解这些肿瘤在活生物体中的病因和发展。目标1.我们将通过基因靶向方法产生具有在DSRCT和尤文肉瘤中发现的类似染色体易位的这些癌症的小鼠模型。然后将成功靶向的小鼠ES细胞注射以产生携带这些染色体易位的小鼠。目标2.我们将检查小鼠自发性肿瘤的形成，并通过与其他癌症易感动物模型交叉引入二次突变，以期加速肿瘤发生。目标3。我们将从动物或形成的肿瘤中产生表达EWS/WT 1和EWS/Fli-1易位的细胞系，并进行表达谱分析以及蛋白质组学（质谱法），以确定致瘤途径和驱动转化的相互作用蛋白。我们的小鼠模型将有助于阐明这些儿科癌症的分子机制，所获得的知识也将有助于了解具有类似特征的其他成人肿瘤。我们的模型也将是非常宝贵的新疗法的发展对DSRCT和尤文肉瘤。