DEVELOPMENT OF A COMMERCIAL POINT-OF-CARE DEVICE CAPABLE OF ELECTRONICALLY SENSI

开发具有电子传感能力的商业护理点设备

• 批准号: 8341485
• 负责人: GARY FUJII
• 金额: $ 19.77万
• 依托单位: MOLECULAR EXPRESS, INC.
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-09-15 至 2012-06-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8341485
• 关键词: Affinity; Antibodies; Architecture; Area; Bacteriophages; Binding; Biological Assay; Biological Markers; Biosensor; Blood; Buffers; Cancer Patient; Cancer cell line; Cell surface; Cells; Detection; Development; Devices; Diagnosis; Digit structure; Electrodes; Engineering; Feedback; Gene Order; Glutamate Carboxypeptidase II; Goals; Housing; Kelp; Laboratories; Ligand Binding; Ligands; Liquid substance; Malignant neoplasm of prostate; Molecular; Nanotechnology; Phage Display; Phase; Prostate-Specific Antigen; Proteins; Research; Scheme; Sensitivity and Specificity; Solutions; Specificity; Testing; Time; Whole Blood; Work; base; cancer cell; commercialization; detector; echistatin; expression vector; forest; improved; neoplastic cell; particle; peripheral blood; point of care; professor; prostate stem cell antigen; prototype; repository; research study; sensor

The long term goal of this proposal is the development of a commercial point-of-care device capable of electronically sensing single circulating tumor cells (CTC) in the peripheral blood of a cancer patient. The viratrode is a nanotechnology platform that represents a significant technological advance. It contains an electrode-based biosensor and can provide an inexpensive format for point-of-care diagnosis with high sensitivity. The viratrode incorporates a bioaffinity layer consisting of a ¿kelp forest¿ of filamentous phage particles, in which each particle can display multiple ligands to a particular cancer cell surface marker. This format maximizes specificity and affinity, while minimizing biofouling. The viratrode incorporates a sensitive area detector capable of detecting single digit or lower nM concentrations of biomarkers. Our Technical Objectives focus on constructing viratrodes for detecting prostate cancer CTC¿s. Our target markers include PSA, PSMA, PSCA, and vβ3 intergrins. The viratrodes will be optimized for the recognition and real-time electrical detection of prostate cancer cells that express the target markers in a biologically relevant fluid matrix.

该提案的长期目标是开发一种商业点护理设备，能够电子感应癌症患者外周血中的单个循环肿瘤细胞（CTC）。viratrode是一种纳米技术平台，代表了重大的技术进步。它包含一个基于电极的生物传感器，可以为高灵敏度的即时诊断提供一种廉价的格式。viratrode结合了一个由丝状噬菌体颗粒组成的“海带森林”组成的生物亲和层，其中每个颗粒可以显示特定癌细胞表面标记物的多个配体。这种形式最大限度地提高了特异性和亲和力，同时最大限度地减少了生物污染。viratrode包含一个敏感区域检测器，能够检测个位数或更低nM浓度的生物标志物。我们的技术目标是构建用于检测前列腺癌CTC的病毒载体。我们的目标标记包括PSA， PSMA， PSCA和&#61537；v&#946；3整合蛋白。viratrodes将被优化用于识别和实时电检测在生物学相关液体基质中表达目标标记的前列腺癌细胞。