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New shRNA vectors for mammalian genetic analysis

New shRNA vectors for mammalian genetic analysis
用于哺乳动物遗传分析的新 shRNA 载体
批准号：
8349457
负责人：
Ji Luo
金额：
$14.14万
依托单位：
DIVISION OF BASIC SCIENCES - NCI
依托单位国家：
美国
项目类别：
财政年份：
--
资助国家：
美国
项目状态：
未结题
起止时间：
至
来源：
https://reporter.nih.gov/project-details/8349457
关键词：
Animal ModelBiologyCaenorhabditis elegansCell LineCellsComplementary DNADrosophila genusGeneticGenotypeGoalsHumanMalignant NeoplasmsMammalian CellMammalian GeneticsMethodsModelingMutationRNA InterferenceSystemTechnologyTumor Suppressor GenesTumor Suppressor ProteinsYeastscDNA Expressiondesignexpression vectorgenetic analysissmall hairpin RNAtoolvector

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中文摘要
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英文摘要
BACKGROUND Genetic analysis in model organisms such as yeast, C. elegans and fruit flies have resulted in many fundamental discoveries in basic biology. The lack of genetic tools in mammalian system has significantly hampered genetics studies in mammalian cells. The advent of RNAi technology have now made it possible to conduct such analysis. PURPOSE We aim to develop new mammalian vector systems to express shRNAs and cDNAs efficiently. This will enable us to introduce multiple, defined genetic changes into cells to create the desired genotype. SIGNIFICANT MATERIALS AND METHODS Expression vectors for shRNAs and cDNAs. FY2011 ACCOMPLISHMENT We have established a design for the vector and validated shRNAs against tumor suppressors.
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Journal of Integrative Plant Biology
  • 批准号：
    31024801
  • 项目类别：
    专项基金项目
  • 资助金额：
    24.0万元
  • 批准年份：
    2010
  • 负责人：
    贺萍
  • 依托单位：
    中国科学院植物研究所