ELECTRON PARAMAGNETIC RESONANCE (EPR) SPECTROSCOPY FACILITY

电子顺磁共振 (EPR) 光谱设备

• 批准号: 8360536
• 负责人: Matthew C. Zimmerman
• 金额: $ 1.35万
• 依托单位: UNIVERSITY OF NEBRASKA LINCOLN
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-08-01 至 2012-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8360536
• 关键词: Biological; Biology; Blood; Cell Culture Techniques; Culture Media; Cultured Cells; Electromagnetic Fields; Electron Spin Resonance Spectroscopy; Electrons; Free Radicals; Funding; Grant; Housing; Hydroxyl Radical; Hydroxylamine; Laboratories; Measures; National Center for Research Resources; Oxidation-Reduction; Physiology; Principal Investigator; Research; Research Infrastructure; Resources; Sampling; Scanning; Source; Superoxides; Tissues; United States National Institutes of Health; absorption; base; cost; microwave electromagnetic radiation

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. EPR (also referred to as Electron Spin Resonance) spectroscopy is based on absorption of microwave energy by free radicals or paramagnetic species in the presence of an external electromagnetic field resulting in the transition of electron spin states. Dr. Zimmerman's laboratory in the Department of Cellular and Integrative Physiology at UNMC houses a Bruker E-Scan Table Top EPR spectrometer (see figure). In combination with EPR-detectable, radical-sensitive cyclic hydroxylamines, often referred to as "spin probes", the E-Scan can be and has been used to measure levels of free radicals (e.g. superoxide, hydroxyl radical) in various biological samples including cultured cells, culture media, blood, and isolated tissue.

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 EPR（也称为电子自旋共振）光谱基于在外部电磁场存在下自由基或顺磁性物质对微波能量的吸收，从而导致电子自旋状态的转变。齐默尔曼博士在UNMC细胞和综合生理学系的实验室拥有布鲁克电子扫描台式EPR光谱仪（见图）。结合EPR可检测的自由基敏感的环羟胺（通常称为“自旋探针”），E-Scan可以并且已经用于测量各种生物样品（包括培养细胞、培养基、血液和分离组织）中的自由基（例如超氧化物、羟基自由基）水平。