BLUETONGUE VIRUS

蓝舌病毒

• 批准号: 8361131
• 负责人: POLLY ROY
• 金额: $ 1.23万
• 依托单位: BAYLOR COLLEGE OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-01-01 至 2011-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8361131
• 关键词: Caliber; Capsid; Cattle; Complex; Decontamination; Electron Microscopy; Funding; Future; Gel; Genetic Transcription; Genome; Goat; Grant; Human; Insect Vectors; National Center for Research Resources; Nucleosome Core Particle; Preparation; Principal Investigator; Proteins; Research; Research Infrastructure; Resources; Ruminants; Sampling; Sheep; Sodium Dodecyl Sulfate-PAGE; Source; Ultracentrifugation; United States National Institutes of Health; Virion; Virus; cost; macromolecule; particle; prevent

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The BTV-1 cores are double-layered (two protein layers) and are ~70nm diameter. There may be some contaminating single-layered particles which are ~52nm diameter, and these lack the VP7 protein. The transcription complexes are located inside the inner layer at the 5 fold symmetry axes (although possibly not at all 12 - the genome consists of just 10 segments). The sample appears to be 100% pure on an SDS PAGE gel and is ~0.1-0.2 mg/ml. Much higher concentrations can be achieved by ultracentrifugation of a larger preparation in the future if necessary. Storage at 4C for months is possible. The core particles (lacking the outer capsid) are much more stable than the complete virion (and easier to purify). The virus is not hazardous to humans, but decontamination with bleach is essential to prevent the potentially infectious cores from infecting the insect vector (which exists in the US) or directly coming into contact with sheep, cows, goats, or wild ruminants.

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 BTV-1核心是双层的（两个蛋白层），直径约为70 nm。可能存在一些直径约52 nm的污染性单层颗粒，并且这些颗粒缺乏VP 7蛋白。转录复合体位于内层的5重对称轴（尽管可能根本不是12 -基因组仅由10个片段组成）。 样品在SDS PAGE凝胶上显示为100%纯，约为0.1-0.2 mg/ml。如有必要，将来可通过超浓缩更大的制剂来达到更高的浓度。 可以在4C下储存数月。核心颗粒（缺少外壳）比完整的病毒粒子稳定得多（并且更容易纯化）。 该病毒对人类无害，但用漂白剂去污是必不可少的，以防止潜在的传染性核心感染昆虫载体（存在于美国）或直接接触绵羊，奶牛，山羊或野生反刍动物。