BLUETONGUE VIRUS

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 BTV-1核心为双层(两层蛋白质层)，直径约70 nm。可能有一些直径约52 nm的污染单层颗粒，这些颗粒缺乏VP7蛋白。转录复合体位于内层的5个对称轴上(尽管可能根本不是12个-基因组只有10个片段)。 样品在SDS PAGE凝胶上显示为100%纯度，约为0.1-0.2 mg/ml。如果需要，未来可以通过超速离心法获得更大规模的制剂，以获得更高的浓度。在4摄氏度下储存数月是可能的。核心粒子(没有外壳)比完整的病毒粒子更稳定(也更容易提纯)。 该病毒对人类无害，但使用漂白剂进行净化是必不可少的，以防止潜在的感染核心感染昆虫媒介(存在于美国)或直接接触绵羊、奶牛、山羊或野生反刍动物。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The BTV-1 cores are double-layered (two protein layers) and are ~70nm diameter. There may be some contaminating single-layered particles which are ~52nm diameter, and these lack the VP7 protein. The transcription complexes are located inside the inner layer at the 5 fold symmetry axes (although possibly not at all 12 - the genome consists of just 10 segments). The sample appears to be 100% pure on an SDS PAGE gel and is ~0.1-0.2 mg/ml. Much higher concentrations can be achieved by ultracentrifugation of a larger preparation in the future if necessary. Storage at 4C for months is possible. The core particles (lacking the outer capsid) are much more stable than the complete virion (and easier to purify). The virus is not hazardous to humans, but decontamination with bleach is essential to prevent the potentially infectious cores from infecting the insect vector (which exists in the US) or directly coming into contact with sheep, cows, goats, or wild ruminants.